Figure 3
Predicted fatty acid and TAG biosynthetic pathways in P. fendleri seeds. Numbers after the gene are the numbers of isotigs for the corresponding genes in the 454 analysis of the seed cDNA library. The dashed arrows in the plastid fatty acid biosynthetic pathway indicate one or more cycles of acyl-chain elongation that is initiated by 3-ketoacyl-ACP synthase (KAS) I or II. The yellow circle delineates reactions in acyl-CoA pools. The bright green rectangle delineates reactions involving desaturation or hydroxylation of PC. The acyl-CoA dependent Kennedy pathway is indicated with red arrows. PC-mediated TAG synthesis pathways are indicated with green (via PDAT), blue (via PDCT) and orange (via LPCAT) arrows. Abbreviations: BCCP, biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase; BC, biotin carboxylase subunit of acetyl-CoA carboxylase; α-CT, α-carboxyltransferase subunit of acetyl-CoA carboxylase; ACP, acyl carrier protein; MCMT, malonly-CoA ACP transferase; KAR, 3-ketoacyl-ACP reductase; HAD, 3-hydroxyacyl-ACP dyhydratase; ER, 2-enoyl-ACP reductase; FAB2/SAD, stearoyl-ACP desaturase; FatA, acyl-ACP thioesterase A; FatB, acyl-ACP thioesterase B; LACS, long-chain acyl-CoA synthase; FAH12, Δ12 oleic acid hydroxylase; FAD2, Δ12 oleic acid desaturase; FAD3, Δ15 (ω-3) linoleic acid desaturase; KCS3, 3-ketoacyl-CoA synthase 3; GPAT9, glycerol 3-phosphate acyltransferase 9 ; LPAT2, lysophosphatidic acid acyltransferase 2; PAPase: phosphatidic acid phosphatase; LPCAT, lysophosphatidylcholine acyltransferase 1 and 2; DGAT, diacylglycerol acyltransferase1 and 2; PDAT, phospholipid:diacylglycerol acyltransferase; G3P, glycerol-3-phosphate; LPA, lysophosphatidic acid; PA, phosphatidic acid; LPC, lysophosphatidylcholine; PC, phosphatidylcholine; DAG, diacylglycerol; TAG, triacylglycerol.