Figure 5

Characterization of two PfFAD3 . (A) Amino acid sequences alignment among Arabidopsis FAD3 (AtFAD3:At2g29980), PfFAD3-1 (isotig17127) and PfFAD3-2 (isotig00061). Black shading indicates identical amino acids. No shading indicates dissimilar amino acids. Dashes indicate gaps in alignment. (B) Phylogenetic tree showing relationships among FAD3 and FAD7/8 desaturases. Lesquerella sequences are shaded. This tree was constructed by DNASTAR MegAlign program using the ClustalW method. Bootstrap used trials = 1000, seed = 111. At, Arabidopsis, Bn, Brassica napus FAD3a (AFJ19040), FAD3b (AFJ19037) and FAD3c (AFJ19034). Arabidopsis FAD7 (At3g11170) and FAD8 (At5g05580) used for outgroup. Expression of PfFAD3-1 (C) and PfFAD3-2 (D) in developing seeds and major organs determined by qPCR. Abbreviated names for the genes are described in Figure 3. Each data point represents the mean ± SD of three replicates. ND = not detected.