Figure 3
From: Natural variation of gene models in Drosophila melanogaster
Genotype-specific alternative donor site associated with cis -sQTL in canonical splicing dinucleotide. De novo donor site and inclusion of an 84 bp segment that was associated with cis-sQTL in canonical splicing dinucleotide. a-b: Exon/intron coverage plots of the genotype-specific splicing event are shown for the F1-hybrids that carried derived (a) and ancestral (b) variants of cis-sQTL on natural alleles. Tester alleles of all F1-hybrids carried ancestral variant of cis-sQTL. c: The distributions of Ψ-values for alleles of this cis-sQTL. d: RT-PCR validation of gene models. The predicted lengths of PCR-products corresponding to the long and short isoforms were 234 bp and 150 bp, respectively. For other details see the legend to Figure 2.