Example of a teleost-specific paralogue phylogenetic and digital expression analysis in pacu fast and slow skeletal muscle. (A) Phylogenetic analysis of the adrb2 teleost-specific paralogues. Human ADRB2 was used as an out-group. (B)
adrb2a and adrb2b digital gene expression. Digital gene expression is represented using a logarithmic scale for slow (full bars) and fast (empty bars) skeletal muscle. Significant differences for paralogues within (***; FDR < 0.001) and between (#; FDR < 0.05) fibre types are indicated. Values represent mean ± SE (n = 5). Adrb2: beta-2 adrenergic receptor.