Relative gene expression in response to fasting and satiation feeding in pacu juveniles in fast skeletal muscle. (A) Heat map summary and hierarchical cluster for all genes analysed in fast skeletal muscle during transition from 4 days of fasting fasting (−4, −3, −2 and −1) to 24 h of satiation feeding (0, 6, 12 and 24 hours). Rows are standardized to have a mean of 0 and standard deviation of 1. Red indicates high and green low expression values. Relative gene expression graphs for mafbx (full circles) and fbox25 (empty circles) (B)
murf1a (full circles) and murf1b (empty circles) (C)
usp2a (full circles) and usp2b (empty circles) (D)
usp12a (full circles), usp12b (empty circles) and usp46 (inverted triangles) (E). Values represents mean ± SE (n = 8). Usp: ubiquiting specific peptidase; fbox: f-box only protein; mafbx: f-box only protein 32; murf1: E3-ubiquitin ligase TRIM63.