Figure 3

Cloning of BAC1 into the iREX. (a) One-step cloning of BAC1 into the iREX. Nm^S, neomycin sensitive; Nm^R, neomycin resistant; Spc^S, spectinomycin sensitive; Spc^R, spectinomycin resistant; I, I-PpoI recognition sequence. (b) iREX/BAC1 was digested with I-PpoI followed by CHEF gel electrophoresis. The BAC1 insert is indicated as an open arrowhead, and the BGM vector is indicated as a closed arrowhead. A lambda DNA concatemer was used as a size marker in lane M. (c) Original BAC1 and genomic DNA of the iREX recombinant were digested with EcoRI or HindIII and hybridized with the original BAC1 clone as a probe. Band patterns identical to the original BAC1 clones were confirmed in the iREX recombinant, except for the bands derived from the BAC end sequences. The closed arrowhead indicates a BAC1-specific signal, and the open arrowheads indicate BGM-specific signals. In lane M, lambda/HindIII fragments were used as a size marker.