De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato

Table 5 Hypericum matches to the plant reproductive database of Arabidopsis

Unigenes	At loci	Biological terms	APO		SEX		Common
			1 repl.	2 repl.	1 repl.	2 repl.
20	9	Cell specification	0	1	1	2	16
90	36	Regulation of megasporogenesis	3	2	16	1	68
9	4	Regulation of megagametogenesis	1	0	0	0	8
79	24	Female gametogenesis, fertilization, seed development	3	0	21	2	53
21	1	Pollen tube reception	0	0	3	0	18
26	10	Seed development and size regulation	5	1	3	0	17
9	3	Imprinted and imprinting related genes	0	0	0	2	7
54	16	Sporophytic mutants with gametophytic defects	1	2	3	3	45
7	2	Gametophytic factor	0	0	2	1	4
25	10	Unfertilized embryo sac	2	1	1	0	21
93	36	Maternal effect embryo arrest	5	1	10	2	75
482	164	Embryo defective	30	2	43	8	399
81	29	Meiosis	8	1	15	6	51
521	183	Gametogenesis	37	3	50	5	426
157	49	Embryogenesis	6	3	19	4	125
1,674	576	Total	101	17	187	36	1,333

The reproductive database was built upon TAIR and NCBI annotations and provides information on genes involved on reproductive processes. For each biological term or process, the number of Hypericum unigenes having a significant match with entries on the reproductive database is shown (unigenes). The number of reference Arabidopsis genes matched by a Hypericum unigene are also reported (At loci). For each biological term or process, the distribution of the Hypericum unigenes among the apomictic and sexual 454 libraries are shown. APO: present only in apomictic libraries; SEX: present only in sexual libraries; Common: present in both apomictic and sexual libraries. 1 repl: detected from a single library; 2 repl: detected in either apomictic (APO) or sexual (SEX) libraries.

ISSN: 1471-2164