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Table 6 Unigenes involved in key biological steps of ovule, gamete and seed formation

From: De novo sequencing of the Hypericum perforatum L. flower transcriptome to identify potential genes that are related to plant reproduction sensu lato

Unigene

Gene

Biological process

DB source

Libraries of flower parts

Apomictic libraries

Sexual libraries

    

C

S/P

St

YB

mean (st.dev.)

mean (st.dev.)

isotig28792

HpANT

Sporophytic mutants with gametophytic defects and seed development and size regulation

2; 8

1.5

0.0

0.0

3.0

0.0 (0. 0)

0.9 (0.0)

isotig03676

HpBEL1

Sporophytic mutants with gametophytic defects

2

2.1

0.0

0.0

0.0

3.1 (0.4)

1.8 (0.2)

isotig24625

HpINO

Sporophytic mutants with gametophytic defects

2

10.5

0.0

0.0

0.0

4.4 (0.0)

2.1 (0.3)

isotig30848

HpAGO9

Cell specification and regulation of megasporogenesis

1; 3; 4

0.0

2.3

0.0

2.4

4.2 (3.4)

0.0 (0.0)

isotig08685

HpRBR

Cell specification and regulation of megagametogenesis

1; 3; 4; G; T

0.0

0.0

0.0

0.0

0.0 (0.0)

2.5 (0.3)

isotig21033

HpRDR6

Regulation of megasporogenesis

3; 4

2.5

1.4

1.1

5.5

3.9 (4.5)

2.1 (0.0)

isotig18046

HpSGS3

Regulation of megagametogenesis

3; 4

8.5

7.1

6.7

8.1

5.3 (0.7)

6.4 (5.5)

isotig27635

HpATREC8

Cell specification and plant meiotic genes

1; 6; T

0.5

0.0

3.1

3.9

0.0 (0.0)

1.9 (1.5)

isotig06846

HpDMC1.1

Plant meiotic genes

6; T

1.0

0.0

2.6

3.4

1.7 (1.3)

3.5 (1.0)

isotig06845

HpDMC1.2

Plant meiotic genes

6; T

1.8

0.0

3.0

4.1

1.9 (1.7)

4.1 (1.5)

isotig30894

HpMND1

Plant meiotic genes

6; G; T

3.1

0.0

0.0

1.2

6.9 (6.4)

1.1 (0.1)

isotig31045

HpMS5

Plant meiotic genes

6; T

2.5

0.0

2.1

0.0

9.3 (10.6)

0.0 (0.0)

isotig32956

HpAGP18

Female gametogenesis, fertilization, seed development

5

2.5

0.0

0.0

0.0

8.1 (9.3)

1.4 (1.9)

isotig10679

HpAGO5.1

Regulation of megagametogenesis

7

3.7

0.6

6.1

2.0

4.6 (1.9)

6.6 (3.4)

isotig15447

HpAGO5.2

Regulation of megagametogenesis

7

5.4

13.0

6.6

8.2

4.4 (1.0)

4.6 (3.5)

isotig24484

HpMET1

Regulation of megagametogenesis and seed development

3; 4; 8

0.7

0.6

0.8

2.6

3.1 (1.7)

2.0 (1.1)

isotig09834

HpDME.1

Imprinted genes and imprinting related

3; 4

0.8

0.0

5.3

0.4

0.0 (0.0)

3.9 (2.7)

isotig09835

HpDME.2

Imprinted genes and imprinting related

3; 4

0.9

0.0

6.3

0.4

0.0 (0.0)

4.6 (3.3)

isotig31020

HpMYB98

Regulation of megagametogenesis, fertilization, seed development

3; 4; 5; T

0.0

5.8

0.0

0.0

0.6 (0.8)

0.6 (0.8)

isotig23549

HpDVPE

Female gametogenesis, fertilization, seed development

5

0.0

0.0

2.1

0.0

2.6 (0.5)

3.9 (1.2)

isotig33211

HpFUS2

Female gametogenesis, fertilization, seed development

5

0.0

0.0

1.0

0.0

0.0 (0.0)

6.1 (8.6)

isotig25989

HpILA

Female gametogenesis, fertilization, seed development

5

11.2

9.4

7.5

3.7

6.9 (1.2)

5.5 (6.8)

isotig23192

HpAGL32

Female gametogenesis, fertilization, seed development

5

14.3

0.0

0.0

0.0

7.9 (1.9)

6.4 (2.1)

isotig11347

HpSERK1

Gametogenesis

T

5.1

0.4

1.9

2.1

5.8 (1.0)

2.7 (0.2)

isotig11348

HpSERK2

Gametogenesis

T

2.9

2.5

1.8

5.0

5.0 (2.3)

3.2 (0.2)

isotig32841

HpIAA32

Maternal effect embryo arrest and gametogenesis

G; T

0.8

0.0

0.0

0.0

4.8 (0.3)

0.0 (0.0)

isotig05393

HpARF2

Seed development and size regulation

8

2.3

2.3

4.1

3.0

3.9 (0.4)

7.5 (0.0)

isotig29668

HpAGL62

Seed development and size regulation

8

0.0

0.0

0.6

0.5

2.2 (1.7)

1.0 (0.1)

isotig29604

HpTANMEI

Embryo defective phenotype and embryogenesis

G; T

2.7

0.0

0.0

0.5

1.4 (0.6)

0.0 (0.0)

isotig25721

HpAHP4

Seed development and size regulation

8

0.0

2.1

0.0

0.0

8.0 (1.3)

18.6 (13.1)

  1. Genes were selected on the basis of their annotation. The number of reads, expressed as RPK (number of reads per kilobase of the reference CDS, normalized over the total number of sequences produced in the specified library and expressed as thousands of sequences), is displayed for each flower parts and for the two reproductive strategies (DB sources: 1, [12]; 2, [11]; 3 [5]; 4 [3]; 5, [4]; 6, [10]; 7, [13]; 8 [8]; G, GenBank; T, TAIR). Unigenes were deposited as TSA project under the accession GBXG00000000.