Expression levels of the read-throughs’ parent genes in tissues. (A) Expression of the BC039389 and GATM parent genes alone (wt, wild-type levels) or with read-throughs (tot, total levels) in 32 frozen tumor and matched normal samples. One sample had not suffient RNA left for measuring wild-type and BC039389 tot levels. The location of the TaqMan assays is indicated in Figure 2A. (B) Expression of the KLK4 and KRSP1 parent genes (wild-type and total transcript levels) in 13 samples expressing KKv1 read-through (left panel) or 19 samples not expressing KKv1 (right panel). To be consistent in showing normal tissues, we artificially calculated KKv1 expression for 11 of 13 normal samples using the cut-off of Ct40, as KKv1 was expressed only in 2 normal tissues of the KKv1-expressing cohort. The location of the TaqMan assays is indicated in Figure 3A. Significance was calculated using student’s t-test in paired samples (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant).