Functional consequences of KLK4-KRSP1 and BC039389-GATM read-throughs on migration and invasion. (A, B) Invasion and migration changes in cell lines under read-through or parent gene knock-down in comparison to si negative control (si nc) (*p < 0.05 by student’s t-test). The migration/invasion of cells was measured in real-time and first plotted as delta cell index (no. of migrated cells) per given time period (see Additional file 1: Figure S7). Then the slopes of the curves of cells treated with targeting siRNAs were calculated in comparison to the baseline si nc curve and plotted as bar chart, where a minus slope indicates less migration, and a positive slope more migration than si nc, respectively. These experiments were performed as technical duplicates in biological replicates as indicated.