Read length vs. GC coverage. This is a representative example of typical read length and coverage (A) of GC rich regions (VEGFA gene) and sequence results imported as custom track in UCSC Genome Browser. Fragments (1 kb) were tiled to cover the entire genomic region of the VEGFA gene (grey bars above sequence). There were a total of 42 amplicons (intragenic sequences totaled 34,268 bp) with 50–150 bp overlap. B. Screen capture showing the high GC content (72%) of the VEGFA gene which was successfully 005B1:1] sequenced.