Figure 3

RT-qPCR analysis of the expression of several nod genes from wild type R. tropici CIAT 899 and nodD1 and nodD2 mutants grown in absence and presence of apigenin (3.7  μM) or NaCl (300 mM). Expression data shown are the mean of three biological replicates. Data were normalized in relation to the endogenous control (16S rRNA). The asterisks indicate a statistically significant expression at the level α = 5%, determined by REST2009 software. Light gray bars: nodD1 mutant, dark gray bars: nodD2 mutant, black bars: wild type strain. A. nodC expression. B. nodD1 expression. C. nodD2 expression.