Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation

Table 1 Nodule number (n° plant ⁻¹ ) and shoot dry weight (g plant ⁻¹ ) of common bean, leucaena and siratro inoculated with R. tropici strain CIAT 899 and nodD derivatives

Strains	*P. vulgaris* ^a		*L. leucocephala* ^a		*M. atropurpureum* ^a
Strains	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight	Nodule number	Shoot dry weight
R. tropici CIAT899	213 ± 52	1.82 ± 0.64	13 ± 4	0.41 ± 0.03	34 ± 8	0.05 ± 0.01
nodD1 mutant	38 ± 11*	1.42 ± 0.35	0 ± 0*	0.09 ± 0.01*	0 ± 0*	0.05 ± 0
nodD2mutant	95 ± 38*	1.03 ± 0.27	10 ± 3	0.36 ± 0.04**	24 ± 8	0.05 ± 0
none	0 ± 0*	0.80 ± 0.25*	0 ± 0*	0.09 ± 0.01*	0 ± 0*	0.05 ± 0

^aData represent means ± SD (standard deviation) of six jars, each with two plants. nodD1 and nodD2 mutant parameters were individually compared with the parental strain CIAT 899 parameters by using the Mann–Whitney non-parametric test. Values tagged by *and **are significantly different at the level α = 5 and 10%, respectively.
Plants evaluated after 25 (common bean) or 42 days (leucaena and siratro) of growth under controlled conditions.

ISSN: 1471-2164