Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng, Hao-Yang; Qi, Yi-Ping; Lee, Jinwook; Yang, Lin-Tong; Guo, Peng; Jiang, Huan-Xin; Chen, Li-Song

doi:10.1186/s12864-015-1462-z

BMC Genomics

Table 1 List of differentially expressed proteins identified using MALDI-TOF/TOF-MS in magnesium (Mg)-deficient Citrus sinensis leaves

From: Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Spot no. ^a	Accession no. ^b	Protein identity	Organism	MW (kDa)	MP/SC	Score	Ratio ^c
*Carbohydrate and energy metabolism*
L118	gi\|4206520	Ribulose 1,5-bisphosphate carboxylase, partial	Severinia buxifolia	49.67	23/86	450	0.267
L20	gi\|19992	Ribulose bisphosphate carboxylase activase	Nicotiana tabacum	25.91	14/92	418	0.478
L24	gi\|290766483	Rubisco activase	Glycine max	52.39	19/90	315	0.051
L26	gi\|19992	Ribulose bisphosphate carboxylase activase	N. tabacum	25.91	14/95	398	0.259
L31	gi\|19992	Ribulose bisphosphate carboxylase activase	N. tabacum	25.91	15/94	435	0.170
L51	gi\|100380	Ribulose-bisphosphate carboxylase activase		25.91	13/96	397	0.388
L52	gi\|19992	Ribulose-bisphosphate carboxylase activase	N. tabacum	25.91	11/98	264	0.190
L48	gi\|326467059	Oxygen evolving enhancer protein 1	Litchi chinensis	35.17	14/96	288	0.020
L115	gi\|89475526	Photosynthetic electron transfer-like protein	Panax ginseng	19.64	5/103	93	0.412
L119	gi\|158145455	Putative ferredoxin-NADP reductase	Solanum peruvianum	18.02	11/98	111	0.325
L114	gi\|77540212	Glyceraldehyde-3-phosphate dehydrogenase B subunit	Glycine max	48.20	13/96	167	0.323
L80	gi\|313585890	Phosphoglycerate kinase	Nicotiana benthamiana	50.05	14/93	357	0.239
L93	gi\|313585890	Phosphoglycerate kinase	N. benthamiana	50.05	11/99	281	0.346
L94	gi\|1161600	Phosphoglycerate kinase	N. tabacum	50.15	20/90	565	0.457
L106	gi\|255567325	Carbonic anhydrase, putative	Ricinus communis	35.64	4/106	83	0.295
L44	gi\|295687231	Triosephosphate isomerase	Gossypium hirsutum	33.10	7/102	121	0
L108	gi\|330252068	Fructose-bisphosphate aldolase, class I	Arabidopsis thaliana	41.78	10/98	138	0.442
L67	gi\|332196500	Putative lactoylglutathione lyase, chloroplast	A. thaliana	39.14	17/82	58	0
L81	gi\|642352	Malate dehydrogenase (NADP)	Spinacia oleracea	47.46	1/98	23	2.320
L89	gi\|255579273	Succinate dehydrogenase, putative	R. communis	68.46	23/87	293	2.438
L104	gi\|285309967	Aconitate hydratase 3	Citrus clementina	98.04	30/78	330	3.176
L112	gi\|255578100	Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase, putative	R. communis	50.84	9/99	115	2.262
L72	gi\|289600010	2-Phospho-D-glycerate hydrolase	Citrus trifoliata	47.76	5/98	449	3.389
L86	gi\|332190370	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase 1	A. thaliana	60.54	20/90	314	3.775
L60	gi\|20336385	Alpha-amylase	Citrus reticulata	17.05	9/101	220	4.083
L77	gi\|7671230	ADP-glucose pyrophosphorylase catalytic subunit	Perilla frutescens	57.44	16/83	63	2.080
L61	gi\|56784991	Putative ATP synthase beta subunit	Oryza sativa Japonica Group	45.88	22/88	706	0.280
L65	gi\|332191230	ATP synthase gamma chain 2	A. thaliana	42.65	7/102	88	0.101
L33	gi\|113952607	ATP synthase CF1 alpha subunit, chloroplastic	C. sinensis	55.45	32/78	781	0.205
L47	gi\|122166198	ATP synthase subunit alpha, chloroplastic		55.45	30/80	632	0.218
L40	gi\|41350585	Putative adenosine kinase	Populus tremula × Populus alba	24.99	6/104	82	2.174
L105	gi\|255571035	Nucleoside diphosphate kinase, putative	R. communis	16.30	3/104	116	3.070
L117	gi\|33149683	Alcohol dehydrogenase	Dianthus caryophyllus	41.23	8/101	284	100
*Protein metabolism*
L79	gi\|255540493	Elongation factor tu, putative	Ricinus communis	50.09	20/90	505	0.357
L25	gi\|806808	Chaperonin precursor (chloroplast)	Pisum sativum	62.95	13/97	177	100
L71	gi\|3098188	Small ribosomal protein 4, partial (chloroplast)	Plagiomnium affine	22.29	12/97	57	24.429
L88	gi\|193788982	Ribosomal protein S3	Trifolium subterraneum	24.57	9/100	51	2.067
L109	gi\|12802327	Mitochondrial processing peptidase beta subunit	Cucumis melo	58.85	17/91	269	100
L41	gi\|332005228	F-box domain-containing protein	A. thaliana	50.19	15/94	57	3.351
L8	gi\|31433129	F-box family protein, putative, expressed	O. sativa Japonica Group	34.58	11/98	57	100
L9	gi\|334302804	Putative F-box/kelch-repeat protein		42.48	16/94	67	100
L62	gi\|12324823	Putative RING zinc finger protein	A. thaliana	12.29	11/99	61	21.438
L4	gi\|89274062	Cysteine proteinase	Platycodon grandiflorus	50.77	6/101	91	3.780
L6	gi\|89274062	Cysteine proteinase	P. grandiflorus	50.77	10/96	63	17.176
L45	gi\|255538698	Proteasome subunit alpha type, putative	R. communis	30.35	11/98	249	3.490
L49	gi\|332656653	Putative cathepsin B-like cysteine protease	A. thaliana	39.32	8/100	148	100
L58	gi\|332656653	Putative cathepsin B-like cysteine protease	A. thaliana	39.32	9/98	156	7.633
L27	gi\|9280680	F2E2.12	A. thaliana	12.88	9/99	55	0.476
L98	gi\|193848487	Putative skp1 protein	Brachypodium distachyon	18.69	10/100	57	0.442
L75	gi\|121489623	Putative glutamine synthetase	P. sativum	39.02	10/99	136	7.333
L91	gi\|121489623	Putative glutamine synthetase	P. sativum	39.02	10/100	196	2.087
L102	gi\|297843044	S-adenosylmethionine synthetase	Arabidopsis lyrata subsp. lyrata	41.43	18/91	230	100
L113	gi\|18150415	Glutathione S-transferase	Allium cepa	23.43	1/96	55	2.873
*Stress responses*
L50	gi\|2274917	Cu/Zn superoxide dismutase	Citrus sinensis	12.78	8/102	259	100
L78	gi\|2274917	Cu/Zn superoxide dismutase	C. sinensis	12.78	6/102	127	22.111
L63	gi\|221327589	Ascorbate peroxidase 2	Citrus maxima	27.56	18/91	230	3.426
L96	gi\|189476292	Ascorbate peroxidase	C. maxima	22.65	10/97	313	100
L97	gi\|186920323	Chloroplast Cu/Zn superoxide dismutase	Hevea brasiliensis	6.92	6/80	219	0.410
L74	gi\|223543700	Aldo/keto reductase, putative	R. communis	37.98	8/99	103	3.418
L103	gi\|255543887	Aldo-keto reductase, putative	R. communis	34.84	5/104	102	7.957
L28	gi\|20559	Heat shock protein 70	Petunia x hybrida	70.74	33/77	523	2.595
L39	gi\|211906496	Heat shock protein 70	Gossypium hirsutum	71.17	34/76	456	3.219
L46	gi\|300265	HSP68 = 68 kda heat-stress DnaK homolog	Lycopersicon peruvianum	62.34	15/94	79	3.960
L59	gi\|259123935	CII small heat shock protein 1	Prunus salicina	17.52	6/10	102	314.482
L76	gi\|116643152	Stress-related protein	Citrus sinensis	17.59	14/94	310	6.476
L90	gi\|116643152	Stress-related protein	Citrus sinensis	17.59	14/95	382	4.825
L116	gi\|332661276	Late embryogenesis abundant (LEA) protein	A. thaliana	37.94	12/98	47	0.441
*Nucleic acid metabolism*
L5	gi\|89258208	Maturase, partial (mitochondrion)	Nepenthes sp. ‘Kosobe’	67.36	16/94	54	3.741
L17	gi\|115466830	Os06g0187000 protein	Oryza sativa Japonica Group	91.71	17/92	56	3.776
L22	gi\|4063759	Mutator-like transposase	A. thaliana	80.331	18/92	65	14.059
L73	gi\|255560725	Dead box ATP-dependent RNA helicase	R. communis	46.812	27/81	393	2.228
*Cell wall and cytoskeleton metabolism*
L16	gi\|56603655	Myosin class 11-1	Adiantum capillus-veneris	173.69	27/82	63	2.339
L21	gi\|216296850	UGT1 (UDP-glucosyltransferase)	Pueraria montana var. lobata	52.19	13/97	56	23.600
L107	gi\|38260664	Pollen coat oleosin-glycine rich protein	Olimarabidopsis pumila	47.00	12/98	39	100
*Cell transport*
L87	gi\|108707728	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein, putative	Oryza sativa Japonica Group	18.36	1/96	34	4.384
L110	gi\|297793335	ATYKT62	A. lyrata subsp. lyrata	22.64	7/52	51	100
*Lipid metabolism*
L43	gi\|108707070	Type I inositol-1,4,5-trisphosphate 5-phosphatase CVP2, putative	Oryza sativa Japonica	54.41	14/94	56	100
L19	gi\|255545978	Cytochrome P450, putative	R. communis	77.12	15/95	48	2.320
*Other and unknown biological processes*
L10	gi\|163943829	Ent-kaurene synthase	Luziola fluitans	42.96	13/96	53	100
L66	gi\|255547472	4-Nitrophenylphosphatase, putative	R. communis	39.69	17/92	167	0
L29	gi\|825532	Orf	Pseudotsuga menziesii	17.00	8/99	51	0.350
L23	gi\|2582665	Thi	C. sinensis	37.57	21/89	197	8.333
L15	gi\|108711987	Streptomyces cyclase/dehydrase family protein, expressed	Oryza sativa Japonica Group	57.34	15/76	60	2.595
L30	gi\|147855631	Hypothetical protein VITISV_019248	Vitis viniferai	35.05	12/98	346	2.365
L64	gi\|302810346	Hypothetical protein SELMODRAFT_182694	Selaginella moellendorffii	47.01	9/101	215	0.480
L42	gi\|147812626	Hypothetical protein VITISV_007608	Vitis vinifera	27.14	8/101	167	11.185
L18	gi\|326530266	Predicted protein	Hordeum vulgare subsp. vulgare	62.53	17/92	59	6.750
L32	gi\|296086060	Unamed protein product	V. vinifera	17.29	18/91	234	100
L7	gi\|296089720	Unnamed protein product	V. vinifera	27.96	7/85	55	0.160
L92	gi\|147821099	Hypothetical protein VITISV_038267	V. vinifera	39.24	11/99	66	3.538

MP/SC: Number of matched peptides/sequence coverage percentage; MW: Theoretical molecular weigh;
^a: Spot number corresponds to the 2-DE gel in Figure 3.
^b: gi number is from NCBI database of matched protein.
^c: Ratio means the ratio of Mg-deficiency to control; 0 means protein spots were only detected in control roots; 100 means protein spots were only detected in the Mg-deficient roots.

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ISSN: 1471-2164

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