Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng, Hao-Yang; Qi, Yi-Ping; Lee, Jinwook; Yang, Lin-Tong; Guo, Peng; Jiang, Huan-Xin; Chen, Li-Song

doi:10.1186/s12864-015-1462-z

BMC Genomics

Table 2 List of differentially expressed proteins identified using MALDI-TOF/TOF-MS in magnesium (Mg)-deficient Citrus sinensis roots

From: Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Spot no. ^a	Accession no. ^b	Protein identity	Organism	MW (kDa)	MP/SC	Score	Ratio ^c
*Carbohydrate and energy metabolism*
R85	gi\|255579310	Pyruvate decarboxylase, putative	Ricinus communis	65.33	11/99	204	0.062
R95	gi\|332195235	Phosphoglycerate kinase	Arabidopsis thaliana	49.91	17/92	110	0.235
R83	gi\|710400	Pyruvate dehydrogenase E1 alpha subunit	A. thaliana	43.00	16/93	56	3.440
R38	gi\|951369	Ferredoxin NADP reductase	Pisum sativum	10.82	8/101	53	2.495
R111	gi\|222356610	ATPase alpha subunit, partial (mitochondrion)	Afrothismia gabonensis	40.27	15/94	210	2.279
R36	gi\|302835814	Adenylate kinase	Volvox carteri f. nagariensis	25.89	11/78	60	2.446
R120	gi\|33149683	Alcohol dehydrogenase	Dianthus caryophyllus	41.23	8/101	287	2.764
R84	gi\|327555177	Beta-amylase 8	Hordeum vulgare subsp. vulgare	51.37	17/93	57	3.121
R121	gi\|11066213	Hexokinase	Citrus sinensis	54.02	14/93	253	6.019
*Protein metabolism*
R1	gi\|255584432	Proteasome subunit alpha type, putative	R. communis	25.99	11/97	190	100
R11	gi\|255620897	Zinc metalloprotease, putative	R. communis	17.94	9/99	57	3.011
R13	gi\|255543801	Cysteine protease, putative	R. communis	41.04	7/103	91	4.924
R35	gi\|332006674	Putative S9 tyrosyl aminopeptidase	A. thaliana	81.26	12/97	73	100
R57	gi\|332006104	Eukaryotic translation initiation factor 3B-2	A. thaliana	82.12	16/93	56	2.402
R34	gi\|806808	Chaperonin precursor	P. sativum	62.95	12/97	131	0.180
R56	gi\|806808	Chaperonin precursor	P. sativum	62.95	19/90	232	0.439
R2	gi\|124484511	Alpha chain of nascent polypeptide associated complex	Nicotiana benthamiana	21.91	10/47	215	0.089
*Stress responses*
R14	gi\|23477636	Grp94 (HSP)	Xerophyta viscosa	92.90	24/84	240	2.990
R70	gi\|227438123	Disease resistance protein	Brassica rapa subsp. pekinensis	81.89	19/90	57	3.881
R53	gi\|399940	Heat shock 70 kDa protein, mitochondrial		72.49	25/84	330	0.319
R100	gi\|4028567	Heat shock protein HSP26	Triticum aestivum	26.48	12/97	66	0.325
*Nucleic acid metabolism*
R37	gi\|90403817	RNA polymerase beta chain	Beaucarnea recurvata	94.49	22/87	72	0.481
R68	gi\|226528292	Spliceosome RNA helicase BAT1	Zea mays	45.12	19/91	245	0.456
R82	gi\|33945882	Transcription factor homolog BTF3-like protein	Lotus japonicus	17.85	11/98	242	0.272
*Cell wall and cytoskeleton metabolism*
R55	gi\|255115691	Actin 1	Boehmeria nivea	41.64	19/90	245	5.833
R69	gi\|71386188	Villin 3	Medicago sativa	20.31	5/104	123	3.134
R101	gi\|225454452	Tubulin gamma-1 chain	Vitis vinifera	53.25	12/97	104	2.600
*Lipid metabolism*
R54	gi\|1117793	Lipoxygenase	Solanum tuberosum	99.60	14/95	65	2.074
R99	gi\|870726	Biotin carboxylase subunit	Nicotiana tabacum	58.35	28/80	419	0.289
*Other and unknown biological processes*
R3	gi\|30017553	Unknown protein, 5’-partial	Oryza sativa Japonica Grou	10.38	5/104	46	2.086
R12	gi\|296086893	Unnamed protein product	Vitis vinifera	9.09	7/101	55	0.278

MP/SC: Number of matched peptides/sequence coverage percentage; MW: Theoretical molecular weigh;
^a: Spot number corresponds to the 2-DE gel in Figure 4.
^b: gi number is from NCBI database of matched protein.
^c: Ratio means the ratio of Mg-deficiency to control; 0 means protein spots were only detected in control roots; 100 means protein spots were only detected in the Mg-deficient roots.

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ISSN: 1471-2164

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