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Table 4 Predicted terminators analysed by qPCR

From: Intrinsic terminators in Mycoplasma hyopneumoniae transcription

Terminator Validation 1 Localisation Class Gene 2 Distance (bp) 3 ΔG 4
w_016 TD mC_04 tc3 rbgA 88 −6.06
w_053 TD mC_15 tc3 pyrG 82 −5.01
w_082 No mC_24 tc3 gyrA 75 −6.41
wf_204 TD mC_30 tc4 pulA 131 −2.48
w_109 TE TU_009 tc2 fusA 39 −17.8
t_022 TD TU_011 tc2 deoA 119 −10
t_055 TE TU_020 tc2 rpmB 49 −7.6
w_140 TD TU_031 tc3 trmE 35 −7.81
w_055 TD TU_047 tc3 ugpQ 18 −8.9
a_207 TD TU_058 tc2 hit 27 −5.8
t_173 TD TU_062 tc2 MHP7448_0372 43 −9.4
wf_140 TD TU_068 tc3 ruvB 42 −3.91
w_181 TD TU_077 tc3 rplA 107 −6.19
a_310 TD TU_083 tc2 MHP7448_0494 24 −5.12
w_093 TD TU_105 tc2 MHP7448_0628 109 −8.35
w_102 TE TU_113 tc2 MHP7448_0665 43 −11
  1. 1Presence of functional activity. TE – transcription termination; TD – transcription decreasing; No – without difference in transcript upstream and downstream of the terminator sequence.
  2. 2Gene at the end of transcription unit.
  3. 3Distance between stop codon of the gene and the first nucleotide of terminator sequence.
  4. 4Value of free energy of Gibbs identified by the software that predicted the terminator.