Three-way comparison of Dichaete binding. (A) Pie chart showing the percentage of all Dichaete binding intervals identified that are present in one species (47%), two species (23%) or all three species (30%). (B) Heatmap showing all biological replicates of Dichaete-Dam samples in D. melanogaster, D. simulans and D. yakuba clustered by binding affinity score (log of normalised read counts) within bound intervals. The color key and histogram shows the distribution of correlation coefficients for affinity scores between each pair of samples. Darker green corresponds to a higher correlation between samples, while lighter green corresponds to a lower correlation. Biological replicates from each species cluster strongly together, though good correlations are seen even between species. (C) Plot of principal component analysis (PCA) of Dichaete-Dam binding affinity scores in bound intervals in D. melanogaster, D. simulans and D. yakuba. The first principal component (x-axis) separates D. yakuba from the other two species, while the second principal component (y-axis) separates D. melanogaster from D. simulans and D. yakuba.