Conservation of lncRNA secondary structure and genomic regions across the Anopheles genus. A. The number of lncRNA secondary structures and conserved genomic regions per-species that are present within members of the Anopheles genus in relation to An. gambiae. Plots represent RNA sequences that possess high confidence (RNAz score > 0.5) secondary structures as identified during REAPR analysis (left, blue line) and conserved genomic regions of the lncRNA gene set (right, red line). For each species in the lineage (phylogenetic tree indicates species), the relative width of the plot corresponds to the number of confident RNA secondary structures or number of conserved genomic regions that were predicted. B. Change in the number of conserved genomic regions (red) and secondary lncRNA structures (blue) over time. Root age of divergence times were determined by Neafsey et al.  and lines represent linear regression. Differences in slopes between the linear regression lines are not significant based upon an ANCOVA test (P-value = 0.09).