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Table 1 Frequencies of cells with aberrant nucleoids and relative genome copy numbers in par mutants and ParAm/ParBm overexpression strains

From: Characterization of chromosomal and megaplasmid partitioning loci in Thermus thermophilus HB27

Strain Cells with aberrant nucleoids (%) Relative TT_P0043 copies Relative term copies Relative oriCc copies Relative terc copies
WT 1.24 1 1 1 1
ΔparABc 3.05 1.13 ± 0.04 1.09 ± 0.05 1.13 ± 0.15 1.14 ± 0.08
ΔparAmN-1 33.02 / 1.52 ± 0.05 0.91 ± 0.13 0.93 ± 0.23
ΔparAmN-2 2.28 1.24 ± 0.09 1.26 ± 0.16 1.08 ± 0.07 1.12 ± 0.02
TMP01 1.26 3.06 ± 0.23 2.45 ± 0.19 0.93 ± 0.17 0.85 ± 0.12
TMP02 2.12 2.84 ± 0.14 2.29 ± 0.35 0.92 ± 0.09 0.89 ± 0.07
TMP0 1.15 0.84 ± 0.26 0.95 ± 0.05 0.97 ± 0.07 1.05 ± 0.03
  1. The frequencies of cells with aberrant nucleoids were measured in cultures grown in TB medium; approximately 300 cells were analyzed for each strain. Relative genome copy numbers were determined by quantitative PCR. The mean values and the standard deviations of three independent experiments are shown. “/” indicates undetectable.