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Table 1 Frequencies of cells with aberrant nucleoids and relative genome copy numbers in par mutants and ParAm/ParBm overexpression strains

From: Characterization of chromosomal and megaplasmid partitioning loci in Thermus thermophilus HB27

Strain

Cells with aberrant nucleoids (%)

Relative TT_P0043 copies

Relative term copies

Relative oriCc copies

Relative terc copies

WT

1.24

1

1

1

1

ΔparABc

3.05

1.13 ± 0.04

1.09 ± 0.05

1.13 ± 0.15

1.14 ± 0.08

ΔparAmN-1

33.02

/

1.52 ± 0.05

0.91 ± 0.13

0.93 ± 0.23

ΔparAmN-2

2.28

1.24 ± 0.09

1.26 ± 0.16

1.08 ± 0.07

1.12 ± 0.02

TMP01

1.26

3.06 ± 0.23

2.45 ± 0.19

0.93 ± 0.17

0.85 ± 0.12

TMP02

2.12

2.84 ± 0.14

2.29 ± 0.35

0.92 ± 0.09

0.89 ± 0.07

TMP0

1.15

0.84 ± 0.26

0.95 ± 0.05

0.97 ± 0.07

1.05 ± 0.03

  1. The frequencies of cells with aberrant nucleoids were measured in cultures grown in TB medium; approximately 300 cells were analyzed for each strain. Relative genome copy numbers were determined by quantitative PCR. The mean values and the standard deviations of three independent experiments are shown. “/” indicates undetectable.
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BMC Genomics

ISSN: 1471-2164

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