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Table 2 Strains and plasmids used in this study

From: Characterization of chromosomal and megaplasmid partitioning loci in Thermus thermophilus HB27

Name Description Source/reference
Plasmids   
pUC18 high-copy-number cloning vector [68]
pMK18 E. coli/T. thermophilus shuttle vector, Tth (repA), Ec (oriE), KmR [63]
pUC-ΔparABc::kat allele exchange vector for generating ΔparABc, ori pUC, KmR this study
pUC-ΔparABm::blm allele exchange vector for generating ΔparABm, ori pUC, BlmR this study
pUC-ΔparAmN-1 allele exchange vector for generating ΔparAmN-1, ori pUC, BlmR this study
pUC-ΔparAmN-2 allele exchange vector for generating ΔparAmN-2, ori pUC, BlmR this study
pUC-ΔparBm::blm allele exchange vector for generating ΔparBm, ori pUC, BlmR this study
pMK-parAm pMK18 derived vector, allowing overexpression of ParAm in Tth this study
pMK-parBm pMK18 derived vector, allowing overexpression of ParBm in Tth this study
pMK-parABm pMK18 derived vector, allowing overexpression of ParABm in Tth this study
pET21a expression vector, PT7, lacI, pBR322 ori, AmpR Novagen, Germany
pET21a-parBc pET21a derived vector, allowing overexpression of ParBc in Ec this study
pET21a-parBm pET21a derived vector, allowing overexpression of ParBm in Ec this study
pMK-sgfp pMK18 derived vector, allowing expression of sGFP in Ec and Tth this study
pMKparBc-sgfp pMK18 derived vector, allowing expression of parBc-sGFP in Ec and Tth this study
pMKparBm-sgfp pMK18 derived vector, allowing expression of parBm-sGFP in Ec and Tth this study
T. thermophilus strains Description Source/reference
HB27 Thermus thermophilus DSM 7039
HB27Δbgl deletion of ORF TT_P0042 in HB27 [69]
TL-1 carotenoid synthesis deficient, otherwise is considered as wild type this study
ΔparABc HB27 derivative with parABc replaced by kat this study
ΔparAmN-1 HB27 derivative with the N-terminal region of parAm replaced by blm in parABm transcription opposite direction this study
ΔparAmN-2 HB27 derivative with the N-terminal region of parAm replaced by blm in parABm transcription co-linear direction this study
HB27/ParABm HB27 derivative carrying a plasmid-borne copy of parABm this study
ΔparAmN-1/ParABm ΔparAmN-1 derivative carrying a plasmid-borne copy of parABm this study
TMP0 HB27 derivative carrying pMK18 vector this study
TMP01 HB27 derivative permitting overexpression of ParAm this study
TMP02 HB27 derivative permitting overexpression of ParBm this study
TL-1/parBc-sGFP TL-1 derivative permitting expression of ParBc-sGFP this study
TL-1/ParBm-sGFP TL-1 derivative permitting expression of ParBm-sGFP this study
ΔparAmN-1/ParBm-sGFP ΔparAmN-1 derivative permitting expression of ParBm-sGFP this study
  1. Tth, T. thermophilus; Ec, E. coli; AmpR, ampicillin resistant; BlmR, bleomycin resistant; KmR, kanamycin resistant; Tth (repA), replication origin for Tth; Ec (oriE), replication origin for Ec; ori pUC, replication origin for pUC18.