Nuclear import of GFP-XYR1 is indistinguishable in QM9136 and QM9414 transformants. (A) Sophorose-induced nuclear import of GFP-XYR1 in QM9136 and QM9414 backgrounds was indistinguishable. Relative fluorescence intensity represents the average GFP-XYR1 signal detected measured in 120 nuclei per sample. The nucleo-cytoplasmic ratio (n/c-ratio) provides a measure of how much brighter the average GFP-XYR1 signal is inside nuclei compared to the surrounding cytoplasm. Error bars represent standard deviation (n = 120). (B) Representative, inverted fluorescence images showing increasing GFP-XYR1 recruitment into nuclei of T. reesei germlings over a time course of 60 min after carbon source replacement with 1.4 mM sophorose.