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Figure 4 | BMC Genomics

Figure 4

From: Digital gene expression approach over multiple RNA-Seq data sets to detect neoblast transcriptional changes in Schmidtea mediterranea

Figure 4

Online data sets and DGE data on Smed454 GBrowse2.A - To facilitate browsing of mapped tags over the transcripts we have worked with, we provide a dynamic table interface that paginates through the huge lists of records. This jQuery [112] interface allows the user to easily sort the output table by a given column—just by clicking on the column label—or to search for specific values on the cells—using either the form box just below the column labels or the advanced search available from the magnifying glass icon at the bottom of the table. Three tables, like the one in the background, contain the equivalences between contigs from different transcriptomes, as well as functional annotations, always focusing on the Smed454 data set. The other tables, like the one in the foreground, contain the tag mappings for each single transcriptomes considered to date. B - Previously published Smed454 database [10] has been ported to GBrowse2 in order to facilitate navigating through the transcripts annotations, such as predicted domains from Pfam, assembly reads mapping, etc. This panel shows the annotations on Smed-wi-3 homologous contig as an example. A customized track allows the integration of information about mapped DGE tags into single or combined tracks; tags are represented as boxes with height proportional to log of the normalized tag counts, the rank and the strand for the tag hit are shown in the label just below that box. Bottom left blue box zooms into one of those combined tracks to visualize the pop-up box that the user can recover when moving the mouse over a given tag feature. In addition, bottom right red box displays the details page one can get when clicking on a tag feature.

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