Figure 5

Splashplot projection of the X1/X2 versus Xin expression changes. X-axis represents tags fold change of X1 with respect to Xin, while Y-axis corresponds to fold change differences between X2 and Xin. Fold change is here calculated as the log base 2 of absolute value of difference between X1, or X2, and Xin, while the direction of the change will be given by the sign of that subtraction. Each of the figure quadrants provide insights on tags expression considering the three cell fractions simultaneously. Upper right quadrant contain tags being overexpressed in both X1 and X2 with respect to Xin; bottom left quadrant has those tags overexpressed in Xin versus the other two fractions. Points over the X-axis or Y-axis correspond to tags for which expression levels change only in one cell fraction, X1 or X2, with respect to Xin. The shift trend on most points towards the right vertical hyperbola reflects a higher expression level in X1 when compared to X2 or Xin (otherwise points will fit closer to both diagonals).