miR-5364 is a novel member of the let-7 miRNA family. A. CLUSTAL alignment comparing miRNA sequences from the C. elegans let-7 family with bpa-miR-5364, bpa-let-7 and bpa-miR-84. Shaded nucleotides are identical. Note the level of identity particularly over the seed sequence (5′ nucleotides 2–7). B. Abundance of bpa-miR-5364, bpa-let-7 and bpa-miR-84 increase in parallel from mosquito-derived (MD) L3 to adult as determined by microarray analysis (log10 scale). The mean of three biological replicates is shown +/− standard deviation.