a) F. hepatica transcripts predicted to be similar to: | Number | Percentage (%) | Data type | Analysis/Database |
Dugesia sp. | 15,784 | 39.21 | Non-parasite-associated | Blast/DDBJ |
Schmidtea sp. | 15,546 | 38.61 | Non-parasite-associated | Blast/DDBJ |
other trematodes (Cura sp., Girardia sp., Neppia sp., Romankenkius sp.) | 524 | 1.30 | Non-parasite-associated | Blast/DDBJ |
Dugesiidae family (in total, excluding common transcripts) | 18,663 | 46.36 | Non-parasite-associated | Blast/DDBJ |
C. elegans | 15,208 | 37.77 | Non-parasite-associated | Blast/WormBase |
C. elegans specific (not associated with Dugesiidae family) | 2,218 | 5.51 | Non-parasite-associated | Blast/WormBase |
Free-living worms (in total) | 20,881 | 51.87 | Non-parasite-associated | Blast/WormBase |
Positive selection related transcripts (Dugesiidae or C. elegans orthologous) | 377 | 1.04 | Virulence-associated | Ka/Ks//DDBJ/WormBase |
Cytokine signaling related transcripts (Dugesiidae or C. elegans orthologous) | 21 | 0.05 | Virulence-associated | InterProScan/EBI |
NVTs (excluding the positive selection and cytokine signaling related transcripts) | 20,483 | 50.88 | Non-parasite-associated | - |
HSD data | 10,694 | 26.56 | Pathogen-associated | Blast/HSD |
Violin data | 1,731 | 4.30 | Pathogen-associated | Blast/Violin |
b) Virulence-related F. hepatica transcripts (VRs) | Number | Percentage (%) | Â | Â |
HTs (HSD related transcripts, not identified as NVTs) | 1,251 | 3.11 | Â | Â |
VTs (Violin related transcripts, not identified as NVTs) | 117 | 0.29 | Â | Â |
HTs/VTs (transcripts homologous to both HSD and Violin data, not identified as NVTs) | 23 | 0.06 | Â | Â |
PDRs (pathogen database related transcripts, including HTs and VTs or HTs/VTs) | 1,391 | 3.46 | Â | Â |
PSRs (positive selection related transcripts; PSR subgroup) | 246 | 0.61 | Â | Â |
CSRs (cytokine signaling related transcripts; CSR subgroup) | 31 | 0.08 | Â | Â |
PSRs and PDRs (common for PSR and PDR subgroups) | 168 | 0.42 | Â | Â |
PSRs and CSRs (common for PSR and CSR subgroups) | 3 | 0.007 | Â | Â |
PDR, PSR and CSR (common for all subgroups) | 1 | 0.002 | Â | Â |
Virulence-related transcripts (VRs) identified as PDRs, PSRs or CSRs | 1,671 | 4.15 | Â | Â |