Mapping of mutations in the intergenic region upstream sa-fabI. The sa-fabI upstream region from the S. aureus Mu50 genome (GenBank ID: BA000017) is reported. Nucleotides in which mutations have been identified are marked in bold. The positions of the mutations are reported with respect to the first nucleotide preceding the start codon of sa-fabI and numbered backwards. The nucleotide substitution is described above the mutation position together with the number of clinical isolates (italicised number) and mutant strains carrying that particular mutation. ATCC6538 sa-fabI upstream region sequence is identical to Mu50, while the naturally occurring polymorphisms identified in the wt strains RN4220 (A92T; GenBank ID: AFGU01000045), ATCC25923 (A213T, A188C; GenBank ID: CP009361), and MW2 (T224A; GenBank ID: BA000033) with respect to the Mu50 sequence are not reported as they do not affect triclosan susceptibility. The putative −35 and −10 consensus sequences, identified by BPROM, are underlined. The consensus of the transcriptional repressor FapR recognition sequence is reported as mapped in RegPrecise (underlined)  or as previously reported by alignment with the experimentally determined one in the fapR upstream region (dotted underlined) [23,24]. The transcriptional start site (+1TSS) as identified by RNAseq  and the ribosomal binding site (SD) are also reported.