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Table 1 Relative expression levels of porcine and bacterial genes

From: Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae

Gene Gene product Control 3 6 h p.i. 3 12 h p.i. 3 24 h p.i. 3 48 h p.i. 3 P value 4
Porcine 1 – Genes involved in pattern recognition
TLR4 Toll-like receptor 4 1.0 ± 0.1 12.6 ± 1.6 8.3 ± 2.6 4.1 ± 1.2 3.7 ± 0.6 9.00E-08
CD14 Cluster of differentiation 14 1.0 ± 0.2 8.7 ± 0.8 7.6 ± 2.1 4.5 ± 1.1 4.1 ± 0.4 8.00E-08
MD2 Myeloid differentiation protein-2 1.0 ± 0.1 3.4 ± 0.4 3.2 ± 0.4 2.0 ± 0.3 2.0 ± 0.4 1.06E-05
LBP Lipopolysaccharide-binding protein 1.0 ± 0.4 8.8 ± 1.9 8.6 ± 4.4 8.6 ± 2.0 24.7 ± 4.5 3.38E-05
MYD88 Myeloid differentiation primary response 88 1.0 ± 0.1 5.2 ± 0.7 4.6 ± 1.5 1.8 ± 0.3 2.5 ± 0.6 7.25E-06
IRF3 Interferon regulatory factor 3 1.0 ± 0.1 1.4 ± 0.2 1.7 ± 0.4 1.0 ± 0.1 1.0 ± 0.1 0.021
SFTPA Surfactant protein A 1.0 ± 0.1 0.6 ± 0.1 0.7 ± 0.2 0.7 ± 0.2 0.3 ± 0.2 0.014
SFTPD Surfactant protein D 1.0 ± 0.1 0.6 ± 0.1 1.0 ± 0.4 0.6 ± 0.1 0.5 ± 0.3 NS
Porcine 1 – Genes involved in involved in iron metabolism     
CD163 CD163 molecule 1.0 ± 0.1 0.8 ± 0.1 3.0 ± 1.1 2.1 ± 0.2 3.0 ± 0.7 0.022
HP Haptoglobin 1.0 ± 0.2 2.2 ± 0.1 2.1 ± 0.2 1.2 ± 0.2 1.2 ± 0.5 0.004
TF Transferrin 1.0 ± 0.1 0.4 ± 0.1 0.5 ± 0.2 0.3 ± 0.1 0.1 ± 0.0 1.31E-04
LTF Lactotransferrin 1.0 ± 0.6 10.2 ± 5.9 30.4 ± 22.0 10.5 ± 6.0 7.1 ± 3.5 NS
Porcine 1 – Cytokines involved in innate immune modulation and inflammation
IL1B Interleukin-1 β 1.0 ± 0.2 414 ± 50 253 ± 93 175 ± 41 209 ± 27 7.20E-07
IL1RN Interleukin-1 receptor antagonist 1.0 ± 0.2 80.1 ± 12.9 53.4 ± 21.2 40.7 ± 9.7 37.8 ± 3.6 7.50E-07
IL6 Interleukin-6 1.0 ± 0.1 1024 ± 67 256 ± 99 80.2 ± 29.1 48.9 ± 22.3 6.00E-08
IL8 Interleukin-8 1.0 ± 0.2 261 ± 19 181 ± 69 91.0 ± 26.1 104 ± 19 1.28E-06
IL17A Interleukin-17A 1.0 ± 0.3 325 ± 54 116 ± 55 46.1 ± 13.4 21.5 ± 4.1 1.49E-05
IL18 Interleukin-18 1.0 ± 0.1 3.7 ± 0.4 7.1 ± 2.8 6.5 ± 2.1 6.0 ± 0.8 1.51E-04
IFNG Interferon-gamma 1.0 ± 0.2 3.1 ± 0.4 1.1 ± 0.3 1.0 ± 0.2 0.3 ± 0.1 1.19E-05
TNF Tumor necrosis factor 1.0 ± 0.1 4.8 ± 0.6 2.5 ± 0.5 2.0 ± 0.7 1.8 ± 0.3 3.20E-04
CSF2 GM-CSF 1.0 ± 0.1 4.4 ± 0.6 2.6 ± 0.9 1.4 ± 0.3 0.6 ± 0.3 4.69E-05
Porcine 1 – Genes involved in the complement system and other functions
C3 Complement component 3 1.0 ± 0.1 1.4 ± 0.1 1.0 ± 0.1 0.9 ± 0.1 0.7 ± 0.2 0.015
CFB Complement factor B 1.0 ± 0.1 5.6 ± 0.4 3.4 ± 0.9 2.7 ± 0.4 2.9 ± 1.1 1.28E-05
CFD Complement factor D 1.0 ± 0.1 1.4 ± 0.2 1.1 ± 0.1 1.1 ± 0.1 0.8 ± 0.1 NS
MASP2 Mannan-binding lectin serine peptidase 2 1.0 ± 0.1 0.4 ± 0.0 0.5 ± 0.2 0.3 ± 0.1 0.2 ± 0.0 1.14E-04
SAA Serum amyloid A 1.0 ± 0.2 15.3 ± 2.3 20.4 ± 11.0 68.9 ± 19.6 110.2 ± 19.6 7.57E-06
TNFAIP3 Tumor necrosis factor, alpha-induced protein 3 1.0 ± 0.1 17.5 ± 1.7 7.7 ± 2.6 4.1 ± 1.0 3.7 ± 0.4 8.00E-07
GZMB Granzyme B 1.0 ± 0.5 3.7 ± 1.6 1.0 ± 0.5 1.3 ± 0.3 0.4 ± 0.1 0.0069
Bacterial 2 – Genes involved in cell wall membrane biogenesis
kdsB 3-deoxy-manno-octulosonate cytidylyltransferase NA 2.6 ± 0.5 1.7 ± 0.2 1.2 ± 0.1 1.0 ± 0.1 7.97E-04
wzxE Lipopolysaccharide biosynthesis protein NA 1.7 ± 0.1 1.4 ± 0.1 1.2 ± 0.1 1.0 ± 0.1 1.50E-03
ompA Outer membrane protein P5 NA 1.0 ± 0.1 2.5 ± 0.3 3.6 ± 0.5 3.6 ± 0.7 3.11E-05
mltC Murein transglycosylase C NA 1.9 ± 0.2 1.7 ± 0.1 1.3 ± 0.1 1.0 ± 0.1 8.20E-05
palA Outer membrane protein NA 1.7 ± 0.1 1.4 ± 0.2 1.0 ± 0.1 1.1 ± 0.1 9.01E-04
tolA Cell envelope integrity inner membrane protein NA 1.0 ± 0.1 1.5 ± 0.1 1.5 ± 0.2 1.6 ± 0.1 9.99E-03
wecC UDP-N-acetyl-D-mannosamine dehydrogenase NA 2.2 ± 0.1 1.8 ± 0.2 1.5 ± 0.1 1.0 ± 0.2 2.72E-04
ompP4 5 Lipoprotein E NA 4.2 ± 0.6 2.8 ± 0.7 1.4 ± 0.3 1.0 ± 0.2 4.07E-04
Bacterial 2 – Genes involved in iron uptake       
afuB Ferric transport system permease protein fbpB NA 3.9 ± 0.4 2.6 ± 0.3 1.6 ± 0.2 1.0 ± 0.1 2.33E-06
hgbA Hemoglobin-binding protein NA 1.3 ± 0.1 1.0 ± 0.1 1.0 ± 0.2 1.0 ± 0.2 NS
Bacterial 2 – Gene involved in exotoxin production      
apxIIA RTX-II toxin determinant A NA 7.0 ± 0.7 3.0 ± 0.7 2.3 ± 0.4 1.0 ± 0.3 5.16E-06
Bacterial 2 – Genes involved in adhesion and competence     
comEA Fibronectin adhesion protein NA 5.8 ± 0.8 2.8 ± 0.5 1.5 ± 0.4 1.0 ± 0.2 5.33E-05
csgG Putative lipoprotein NA 1.2 ± 0.1 1.2 ± 0.2 1.0 ± 0.2 1.0 ± 0.2 NS
Bacterial 2 – Genes other functions       
dnaK Chaperone protein Dna NA 2.9 ± 0.4 2.6 ± 1.3 2.0 ± 0.8 1.0 ± 0.5 NS
mgsA Methylglyoxal synthase NA 1.8 ± 0.2 1.3 ± 0.2 1.0 ± 0.1 1.4 ± 0.2 0.027
  1. 1Sample sizes: Control: n = 7; 6 h p.i.: n = 6; 12 h p.i.: n = 5; 24 h p.i.: n = 8; 48 h p.i.: n = 5.
  2. 2Number of data points: 6 h p.i.: n = 6; 12 h p.i.: n = 4; 24 h p.i.: n = 7; 48 h p.i.: n = 5.
  3. 3Expression is given ± SEM.
  4. 4P values are included for genes that were differentially expressed during the course of the study (ANOVA, P < 0.05). NS = not significant.
  5. 5Gene also involved in iron uptake (heme acquisition).