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Table 1 Relative expression levels of porcine and bacterial genes

From: Concurrent host-pathogen gene expression in the lungs of pigs challenged with Actinobacillus pleuropneumoniae

Gene

Gene product

Control 3

6 h p.i. 3

12 h p.i. 3

24 h p.i. 3

48 h p.i. 3

P value 4

Porcine 1 – Genes involved in pattern recognition

TLR4

Toll-like receptor 4

1.0 ± 0.1

12.6 ± 1.6

8.3 ± 2.6

4.1 ± 1.2

3.7 ± 0.6

9.00E-08

CD14

Cluster of differentiation 14

1.0 ± 0.2

8.7 ± 0.8

7.6 ± 2.1

4.5 ± 1.1

4.1 ± 0.4

8.00E-08

MD2

Myeloid differentiation protein-2

1.0 ± 0.1

3.4 ± 0.4

3.2 ± 0.4

2.0 ± 0.3

2.0 ± 0.4

1.06E-05

LBP

Lipopolysaccharide-binding protein

1.0 ± 0.4

8.8 ± 1.9

8.6 ± 4.4

8.6 ± 2.0

24.7 ± 4.5

3.38E-05

MYD88

Myeloid differentiation primary response 88

1.0 ± 0.1

5.2 ± 0.7

4.6 ± 1.5

1.8 ± 0.3

2.5 ± 0.6

7.25E-06

IRF3

Interferon regulatory factor 3

1.0 ± 0.1

1.4 ± 0.2

1.7 ± 0.4

1.0 ± 0.1

1.0 ± 0.1

0.021

SFTPA

Surfactant protein A

1.0 ± 0.1

0.6 ± 0.1

0.7 ± 0.2

0.7 ± 0.2

0.3 ± 0.2

0.014

SFTPD

Surfactant protein D

1.0 ± 0.1

0.6 ± 0.1

1.0 ± 0.4

0.6 ± 0.1

0.5 ± 0.3

NS

Porcine 1 – Genes involved in involved in iron metabolism

    

CD163

CD163 molecule

1.0 ± 0.1

0.8 ± 0.1

3.0 ± 1.1

2.1 ± 0.2

3.0 ± 0.7

0.022

HP

Haptoglobin

1.0 ± 0.2

2.2 ± 0.1

2.1 ± 0.2

1.2 ± 0.2

1.2 ± 0.5

0.004

TF

Transferrin

1.0 ± 0.1

0.4 ± 0.1

0.5 ± 0.2

0.3 ± 0.1

0.1 ± 0.0

1.31E-04

LTF

Lactotransferrin

1.0 ± 0.6

10.2 ± 5.9

30.4 ± 22.0

10.5 ± 6.0

7.1 ± 3.5

NS

Porcine 1 – Cytokines involved in innate immune modulation and inflammation

IL1B

Interleukin-1 β

1.0 ± 0.2

414 ± 50

253 ± 93

175 ± 41

209 ± 27

7.20E-07

IL1RN

Interleukin-1 receptor antagonist

1.0 ± 0.2

80.1 ± 12.9

53.4 ± 21.2

40.7 ± 9.7

37.8 ± 3.6

7.50E-07

IL6

Interleukin-6

1.0 ± 0.1

1024 ± 67

256 ± 99

80.2 ± 29.1

48.9 ± 22.3

6.00E-08

IL8

Interleukin-8

1.0 ± 0.2

261 ± 19

181 ± 69

91.0 ± 26.1

104 ± 19

1.28E-06

IL17A

Interleukin-17A

1.0 ± 0.3

325 ± 54

116 ± 55

46.1 ± 13.4

21.5 ± 4.1

1.49E-05

IL18

Interleukin-18

1.0 ± 0.1

3.7 ± 0.4

7.1 ± 2.8

6.5 ± 2.1

6.0 ± 0.8

1.51E-04

IFNG

Interferon-gamma

1.0 ± 0.2

3.1 ± 0.4

1.1 ± 0.3

1.0 ± 0.2

0.3 ± 0.1

1.19E-05

TNF

Tumor necrosis factor

1.0 ± 0.1

4.8 ± 0.6

2.5 ± 0.5

2.0 ± 0.7

1.8 ± 0.3

3.20E-04

CSF2

GM-CSF

1.0 ± 0.1

4.4 ± 0.6

2.6 ± 0.9

1.4 ± 0.3

0.6 ± 0.3

4.69E-05

Porcine 1 – Genes involved in the complement system and other functions

C3

Complement component 3

1.0 ± 0.1

1.4 ± 0.1

1.0 ± 0.1

0.9 ± 0.1

0.7 ± 0.2

0.015

CFB

Complement factor B

1.0 ± 0.1

5.6 ± 0.4

3.4 ± 0.9

2.7 ± 0.4

2.9 ± 1.1

1.28E-05

CFD

Complement factor D

1.0 ± 0.1

1.4 ± 0.2

1.1 ± 0.1

1.1 ± 0.1

0.8 ± 0.1

NS

MASP2

Mannan-binding lectin serine peptidase 2

1.0 ± 0.1

0.4 ± 0.0

0.5 ± 0.2

0.3 ± 0.1

0.2 ± 0.0

1.14E-04

SAA

Serum amyloid A

1.0 ± 0.2

15.3 ± 2.3

20.4 ± 11.0

68.9 ± 19.6

110.2 ± 19.6

7.57E-06

TNFAIP3

Tumor necrosis factor, alpha-induced protein 3

1.0 ± 0.1

17.5 ± 1.7

7.7 ± 2.6

4.1 ± 1.0

3.7 ± 0.4

8.00E-07

GZMB

Granzyme B

1.0 ± 0.5

3.7 ± 1.6

1.0 ± 0.5

1.3 ± 0.3

0.4 ± 0.1

0.0069

Bacterial 2 – Genes involved in cell wall membrane biogenesis

kdsB

3-deoxy-manno-octulosonate cytidylyltransferase

NA

2.6 ± 0.5

1.7 ± 0.2

1.2 ± 0.1

1.0 ± 0.1

7.97E-04

wzxE

Lipopolysaccharide biosynthesis protein

NA

1.7 ± 0.1

1.4 ± 0.1

1.2 ± 0.1

1.0 ± 0.1

1.50E-03

ompA

Outer membrane protein P5

NA

1.0 ± 0.1

2.5 ± 0.3

3.6 ± 0.5

3.6 ± 0.7

3.11E-05

mltC

Murein transglycosylase C

NA

1.9 ± 0.2

1.7 ± 0.1

1.3 ± 0.1

1.0 ± 0.1

8.20E-05

palA

Outer membrane protein

NA

1.7 ± 0.1

1.4 ± 0.2

1.0 ± 0.1

1.1 ± 0.1

9.01E-04

tolA

Cell envelope integrity inner membrane protein

NA

1.0 ± 0.1

1.5 ± 0.1

1.5 ± 0.2

1.6 ± 0.1

9.99E-03

wecC

UDP-N-acetyl-D-mannosamine dehydrogenase

NA

2.2 ± 0.1

1.8 ± 0.2

1.5 ± 0.1

1.0 ± 0.2

2.72E-04

ompP4 5

Lipoprotein E

NA

4.2 ± 0.6

2.8 ± 0.7

1.4 ± 0.3

1.0 ± 0.2

4.07E-04

Bacterial 2 – Genes involved in iron uptake

      

afuB

Ferric transport system permease protein fbpB

NA

3.9 ± 0.4

2.6 ± 0.3

1.6 ± 0.2

1.0 ± 0.1

2.33E-06

hgbA

Hemoglobin-binding protein

NA

1.3 ± 0.1

1.0 ± 0.1

1.0 ± 0.2

1.0 ± 0.2

NS

Bacterial 2 – Gene involved in exotoxin production

     

apxIIA

RTX-II toxin determinant A

NA

7.0 ± 0.7

3.0 ± 0.7

2.3 ± 0.4

1.0 ± 0.3

5.16E-06

Bacterial 2 – Genes involved in adhesion and competence

    

comEA

Fibronectin adhesion protein

NA

5.8 ± 0.8

2.8 ± 0.5

1.5 ± 0.4

1.0 ± 0.2

5.33E-05

csgG

Putative lipoprotein

NA

1.2 ± 0.1

1.2 ± 0.2

1.0 ± 0.2

1.0 ± 0.2

NS

Bacterial 2 – Genes other functions

      

dnaK

Chaperone protein Dna

NA

2.9 ± 0.4

2.6 ± 1.3

2.0 ± 0.8

1.0 ± 0.5

NS

mgsA

Methylglyoxal synthase

NA

1.8 ± 0.2

1.3 ± 0.2

1.0 ± 0.1

1.4 ± 0.2

0.027

  1. 1Sample sizes: Control: n = 7; 6 h p.i.: n = 6; 12 h p.i.: n = 5; 24 h p.i.: n = 8; 48 h p.i.: n = 5.
  2. 2Number of data points: 6 h p.i.: n = 6; 12 h p.i.: n = 4; 24 h p.i.: n = 7; 48 h p.i.: n = 5.
  3. 3Expression is given ± SEM.
  4. 4P values are included for genes that were differentially expressed during the course of the study (ANOVA, P < 0.05). NS = not significant.
  5. 5Gene also involved in iron uptake (heme acquisition).
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BMC Genomics

ISSN: 1471-2164

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