Figure 3
From: Fungal artificial chromosomes for mining of the fungal secondary metabolome
Identification of astechrome biosynthetic precursor, terezine D. (a) A compound was identified in FAC 6J7 cell extracts with exact mass matching the theoretical exact mass of terezine D, the predicted product of FAC 6J7. (b) Selected ion chromatograms of lysates from cells containing FAC 6J7 and all other analyzed FACs show that the compound of interest is unique to the FAC 6J7 expressing strain. (c) Analysis of MS2 fragmentation data for the identified compound lead to identification of predicted fragments of terezine D, confirming the compound’s identity.