Translation efficiency (TE) varies between the epimastigote (E) and metacyclic trypomastigote (MT) T. cruzi stages. Upper panel:
) Scatter plot of the fold change (MT/E expression estimates) in the translatome vs the transcriptome. (
) Scatter plot of the TE (RFP/Total RNA expression estimates) in the MT vs the E stage for genes detected in all samples. Genes exhibiting non proportional changes (FC > 2, FDR < 0.05) are colored. Lower panel: (
) TE histograms for epimastigotes (light grey) and metacyclic trypomastigotes (dark grey). Median efficiency values are 0.51 and 0.69 respectively.