Figure 4

Genomic Southern blot analysis and chromosomal distribution of RtPc1 and RtPc5 elements. Genomic DNA from P. lutzii (panels A and D) and the Pb18 P. brasiliensis isolate (panels B and E) were digested with restriction enzymes, blotted onto nylon membranes and hybridized with RtPc1 (panels A and B) and RtPc5 (panels D and E) probes derived from the transcriptase reverse region of each element. The restriction enzymes used were EcoRI (E), BamHI (B), HindIII (H), BglII(Bg), HinfI (Hf), HincII (Hc), AccI (Ac) and EcoRV (Ev). Chromosomal distribution of RtPc1 (panel C) and RtPc5 (panel F) elements in P. lutzii and P. brasiliensis. The molecular karyotypes of P. lutzii and P. brasiliensis (B339 and Pb18) are shown on the left. Chromosomal bands were separated by PFGE and stained with EtBr. The autoradiograms from Southern hybridizations using the RtPc1 and RtPc5 probes derived from the transcriptase reverse region are shown on the right.