K-means clusters showing seasonal and daily patterns of gene expression. A) Representative K-means clusters showing upregulation in all summer sample groups, VMN summer groups, and peak expression in summer night VMN (SNV; see Figure 1C for other abbreviations). Total transcript numbers for each K-means cluster expression pattern are shown next to the cluster type names used in the text. B) FPKM expression levels are plotted for our candidate ion channels exhibiting peak expression in SNV. chrnb2: neuronal acetylcholine receptor subunit beta-2; gabrp: gamma-aminobutyric acid receptor subunit pi; cx30: connexin 30/gap junction beta-6; kcnma1 (or Kca1.1): calcium-activated potassium channel subunit alpha-1.