Figure 3

Connecting a putative quantitative trait nucleotide (QTN) with a candidate quantitative trait gene (QTG). A: A SNP identified in the SM/J strain (chr13:104041257 A→C) falls in a highly informative position (position 2) of a predicted cMyb TFBS. B and C: This SNP falls in an intron of Nln, a protein-coding gene associated with gluconeogenesis. Nln falls in a QTL associated with variation in basal glucose levels in an F16 generation of a LG x SM advanced intercross and is highly significantly differentially expressed between the LG/J and SM/J strains. C: This variant overlaps multiple regulatory elements and is a strong candidate for mechanistic studies of Nln function in glucose metabolism.