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Table 2 Differentially expressed proteins under ex vivo conditions in macrophage model

From: Quantitative proteomic analysis of host—pathogen interactions: a study of Acinetobacter baumannii responses to host airways

Protein description AbH12O-A2 locus Sec.a macrophage model/ controlb macrophage model/ control p-valuec
Pathogenesis and virulence
Nucleotidyl transferase AIS04894.1 NO 2.83 (0.60) 0.013
Trigger factor AIS05263.1 NO 3.37 (0.37) 0.016
ATPase AAA (PaaA) AIS06044.1 NO 5.50 (4.44) 0.028
Siderophore achromobactin biosynthesis proteína AcsC AIS06377.1 NO 0.44 (0.17) 0.000
Cyclophilin (PPIase) AIS06968.1 NO 2.83 (0.87) 0.041
Protein CsuC AIS07073.1 YES 0.16 (0.06) 0.004
Protein CsuA AIS07075.1 YES 0.31 (0.22) 0.040
Metallopeptidase AIS07555.1 YES 0.20 (0.16) 0.028
Membrane protein (OmpA) AIS07737.1 YES 7.80 (2.78) 0.049
Oligopeptidase A AIS07885.1 NO 4.83 (0.88) 0.006
ABC transporter ATP-binding protein (YjjK) AIS08062.1 NO 2.33 (0.77) 0.046
Amino acid metabolism and transport
Aspartate aminotransferase AIS07065.1 NO 0.39 (0.18) 0.010
Serine hydroxymethyltransferase AIS07167.1 NO 2.54 (0.44) 0.043
Carbamoyl-phosphate synthase AIS07544.1 NO 2.75 (1.74) 0.035
4-Hydroxyphenylpyruvate dioxygenase AIS08279.1 NO 4.74 (1.15) 0.000
Carbohydrate metabolism and transport
Phosphoglyceromutase AIS05033.1 NO 3.94 (1.06) 0.032
Glyceraldehyde-3-phosphate dehydrogenase AIS07393.1 NO 3.08 (0.66) 0.016
Cell cycle control and mitosis
Cell division protein FtsA AIS08196.1 NO 4.74 (7.32) 0.002
Cell wall/membrane/envelope biogenesis
Membrane protein AIS06856.1 YES 8.02 (7.90) 0.006
Racemase AIS07059.1 NO 0.20 (0.13) 0.037
UDP-N-acetylmuramate-alanine ligase (MurC) AIS08199.1 NO 8.17 (3.95) 0.040
Coenzyme metabolism
Pantoate-beta-alanine ligase (PanC) AIS05347.1 NO 6.08 (8.59) 0.010
3-Phosphoglycerate dehydrogenase AIS07996.1 NO 3.77 (0.88) 0.014
Defense mechanisms (cellular processes and signaling)
Beta-lactamase AIS07280.1 YES 2.09 (0.41) 0.049
ABC transporter AIS07290.1 NO 0.39 (0.18) 0.037
Energy production and conversion
ATP synthase subunit B AIS04971.1 NO 5.50 (2.54) 0.044
ATP synthase F0F1 subunit beta AIS04975.1 NO 3.63 (0.48) 0.002
Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase AIS06989.1 NO 3.16 (0.31) 0.002
Isocitrate dehydrogenase AIS07371.1 NO 4.57 (0.55) 0.002
Electron transfer flavoprotein subunit beta AIS07482.1 YES 5.65 (0.89) 0.005
Dihydrolipoamide succinyltransferase AIS07561.1 NO 2.44 (0.45) 0.036
Fumarate reductase AIS07564.1 NO 3.08 (0.59) 0.010
Acetyl-CoA hydrolase AIS08069.1 NO 4.74 (1.68) 0.040
NADPH:quinone oxidoreductase AIS08156.1 NO 15.42 (16.20) 0.034
Dihydrolipoamide acetyltransferase AIS08190.1 YES 3.08 (0.73) 0.010
Unknown function
Hypothetical protein AIS05430.1 NO 19.23 (19.19) 0.001
Hypothetical protein AIS05481.1 YES 0.17 (0.08) 0.000
Unknown function
Hypothetical protein AIS05936.1 YES 0.23 (0.13) 0.006
DNA-binding protein AIS07086.1 YES 0.24 (0.17) 0.037
Hypothetical protein AIS07091.1 NO 0.21 (0.12) 0.000
DcaP-like protein AIS07608.1 YES 11.07 (6.85) 0.023
Glyoxalase AIS07740.1 YES 0.30 (0.23) 0.036
Hypothetical protein AIS07873.1 YES 0.42 (0.16) 0.011
Inorganic ion transport and metabolism
Hydroperoxidase AIS06129.1 YES 0.35 (0.13) 0.008
ABC transporter permease AIS07421.1 YES 8.17 (3.60) 0.034
Lipid metabolism
3-Oxoacyl-ACP reductase AIS05519.1 YES 2.68 (0.94) 0.038
Enoyl-CoA hydratase AIS06112.1 NO 0.17 (0.24) 0.004
Short-chain dehydrogenase AIS06130.1 YES 0.10 (0.07) 0.032
Nucleotide metabolism and transport
N5-carboxyaminoimidazole ribonucleotide mutase AIS04832.1 NO 4.02 (2.98) 0.010
Ribose-phosphate pyrophosphokinase AIS05530.1 NO 4.61 (2.76) 0.000
Deoxyuridine 5′-triphosphate nucleotidohydrolase AIS05628.1 NO 0.28 (0.32) 0.032
Orotidine 5′-phosphate decarboxylase AIS06325.1 NO 0.19 (0.21) 0.032
Adenylosuccinate lyase AIS07333.1 NO 6.31 (2.68) 0.047
Xanthine phosphoribosyltransferase AIS07874.1 NO 2.42 (0.69) 0.048
Inosine-5-monophosphate dehydrogenase AIS08184.1 NO 3.94 (0.81) 0.006
Post-translational modification, protein turnover, chaperone functions
Molecular chaperone DnaK AIS04838.1 NO 2.13 (0.15) 0.000
Post-translational modification, protein turnover, chaperone functions
Osmotically inducible protein C AIS04957.1 NO 3.16 (1.14) 0.029
Replication and repair
DNA repair protein AIS04967.1 YES 3.87 (1.19) 0.033
DNA polymerase I AIS05372.1 NO 0.31 (0.34) 0.038
Secondary metabolites: biosynthesis, transport and catabolism
mRNA 3′-end processing factor AIS06680.1 NO 0.10 (0.19) 0.027
Transcription
DNA-directed RNA polymerase subunit beta’ AIS05081.1 NO 2.86 (0.42) 0.025
Transcription elongation factor NusA AIS05126.1 NO 3.53 (0.94) 0.036
Transcription termination factor Rho AIS05365.1 NO 3.60 (1.54) 0.025
DNA-binding protein AIS07045.1 NO 9.73 (6.82) 0.029
DNA-directed RNA polymerase subunit alpha AIS07896.1 NO 3.28 (0.46) 0.001
Translation
Tyrosyl-tRNA synthetase AIS04798.1 NO 3.37 (0.97) 0.009
23S rRNA methyltransferase AIS05117.1 NO 3.37 (4.80) 0.037
Alanyl-tRNA synthetase AIS05862.1 NO 3.05 (1.16) 0.004
30S ribosomal protein S20 AIS06347.1 YES 3.77 (2.31) 0.026
Ribosome-recycling factor AIS06861.1 NO 3.91 (1.42) 0.045
Peptide chain release factor 1 AIS07012.1 NO 0.34 (0.27) 0.021
30S ribosomal protein S6 AIS07031.1 NO 6.92 (1.66) 0.000
30S ribosomal protein S18 AIS07032.1 NO 22.70 (16.56) 0.028
50S ribosomal protein L9 AIS07033.1 NO 8.17 (3.02) 0.002
30S ribosomal protein S2 AIS07184.1 NO 3.87 (1.07) 0.040
Translation
Tryptophanyl-tRNA synthetase AIS07557.1 NO 8.09 (4.24) 0.023
50S ribosomal protein L27 AIS07585.1 YES 10.28 (8.73) 0.027
Aspartyl-tRNA synthetase AIS07787.1 NO 5.92 (1.79) 0.038
30S ribosomal protein S9 AIS07845.1 YES 5.86 (1.38) 0.009
50S ribosomal protein L17 AIS07895.1 YES 9.64 (4.81) 0.003
30S ribosomal protein S4 AIS07897.1 NO 13.30 (3.19) 0.010
50S ribosomal protein L15 AIS07902.1 NO 5.92 (1.17) 0.000
30S ribosomal protein S5 AIS07904.1 YES 4.61 (5.00) 0.003
50S ribosomal protein L18 AIS07905.1 YES 9.04 (10.58) 0.033
50S ribosomal protein L6 AIS07906.1 YES 7.59 (1.58) 0.037
30S ribosomal protein S8 AIS07907.1 YES 10.76 (2.98) 0.011
50S ribosomal protein L5 AIS07909.1 NO 7.73 (2.20) 0.000
50S ribosomal protein L24 AIS07910.1 YES 4.25 (0.70) 0.038
50S ribosomal protein L14 AIS07911.1 NO 11.07 (12.57) 0.002
30S ribosomal protein S17 AIS07912.1 NO 12.36 (3.91) 0.048
50S ribosomal protein L16 AIS07914.1 YES 29.11 (22.95) 0.038
30S ribosomal protein S3 AIS07915.1 NO 6.73 (3.82) 0.026
50S ribosomal protein L22 AIS07916.1 NO 1.89 (0.85) 0.035
50S ribosomal protein L2 AIS07918.1 YES 7.87 (3.98) 0.038
50S ribosomal protein L23 AIS07919.1 YES 4.45 (1.04) 0.018
50S ribosomal protein L4 AIS07920.1 NO 14.45 (7.02) 0.002
Translation
50S ribosomal protein L3 AIS07921.1 NO 6.25 (1.29) 0.042
  1. The protein profiles produced by A. baumannii grown in the presence of macrophages were performed using iTRAQ reagents and LC-MS/MS. Differential expression was defined by a relative abundance ratio >1.5 and <0.5
  2. aSecretion prediction are based on (SignalP 4.1 (http://www.cbs.dtu.dk/services/SignalP/), Phobius (http://phobius.sbc.su.se/), PrediSi (http://www.predisi.de/), TatP 1.0 (www.cbs.dtu.dk/services/TatP-1.0), Tatfind 1.4 (http://signalfind.org/tatfind.html), SecretomeP 2.0 (www.cbs.dtu.dk/services/SecretomeP), TMHMM (http://www.cbs.dtu.dk/services/TMHMM/), DAS-TMfilter (http://www.enzim.hu/DAS/DAS.html), LipoP 1.0 (www.cbs.dtu.dk/services/LipoP), DOLOP (http://www.mrc-lmb.cam.ac.uk/genomes/dolop/), and LIPO (http://services.cbu.uib.no/tools/lipo))
  3. bAverage relative protein expression level ratio in sample and control, with the standard deviation in parentheses, quantified by Protein Pilot 4.0 software (ABSciex).
  4. cDetermined by Student’s t test. Values of less than 0.05 are considered significant