Table 2 Differentially expressed proteins under ex vivo conditions in macrophage model
Protein description | AbH12O-A2 locus | Sec.a | macrophage model/ controlb | macrophage model/ control p-valuec |
|---|---|---|---|---|
Pathogenesis and virulence | ||||
Nucleotidyl transferase | AIS04894.1 | NO | 2.83 (0.60) | 0.013 |
Trigger factor | AIS05263.1 | NO | 3.37 (0.37) | 0.016 |
ATPase AAA (PaaA) | AIS06044.1 | NO | 5.50 (4.44) | 0.028 |
Siderophore achromobactin biosynthesis proteína AcsC | AIS06377.1 | NO | 0.44 (0.17) | 0.000 |
Cyclophilin (PPIase) | AIS06968.1 | NO | 2.83 (0.87) | 0.041 |
Protein CsuC | AIS07073.1 | YES | 0.16 (0.06) | 0.004 |
Protein CsuA | AIS07075.1 | YES | 0.31 (0.22) | 0.040 |
Metallopeptidase | AIS07555.1 | YES | 0.20 (0.16) | 0.028 |
Membrane protein (OmpA) | AIS07737.1 | YES | 7.80 (2.78) | 0.049 |
Oligopeptidase A | AIS07885.1 | NO | 4.83 (0.88) | 0.006 |
ABC transporter ATP-binding protein (YjjK) | AIS08062.1 | NO | 2.33 (0.77) | 0.046 |
Amino acid metabolism and transport | ||||
Aspartate aminotransferase | AIS07065.1 | NO | 0.39 (0.18) | 0.010 |
Serine hydroxymethyltransferase | AIS07167.1 | NO | 2.54 (0.44) | 0.043 |
Carbamoyl-phosphate synthase | AIS07544.1 | NO | 2.75 (1.74) | 0.035 |
4-Hydroxyphenylpyruvate dioxygenase | AIS08279.1 | NO | 4.74 (1.15) | 0.000 |
Carbohydrate metabolism and transport | ||||
Phosphoglyceromutase | AIS05033.1 | NO | 3.94 (1.06) | 0.032 |
Glyceraldehyde-3-phosphate dehydrogenase | AIS07393.1 | NO | 3.08 (0.66) | 0.016 |
Cell cycle control and mitosis | ||||
Cell division protein FtsA | AIS08196.1 | NO | 4.74 (7.32) | 0.002 |
Cell wall/membrane/envelope biogenesis | ||||
Membrane protein | AIS06856.1 | YES | 8.02 (7.90) | 0.006 |
Racemase | AIS07059.1 | NO | 0.20 (0.13) | 0.037 |
UDP-N-acetylmuramate-alanine ligase (MurC) | AIS08199.1 | NO | 8.17 (3.95) | 0.040 |
Coenzyme metabolism | ||||
Pantoate-beta-alanine ligase (PanC) | AIS05347.1 | NO | 6.08 (8.59) | 0.010 |
3-Phosphoglycerate dehydrogenase | AIS07996.1 | NO | 3.77 (0.88) | 0.014 |
Defense mechanisms (cellular processes and signaling) | ||||
Beta-lactamase | AIS07280.1 | YES | 2.09 (0.41) | 0.049 |
ABC transporter | AIS07290.1 | NO | 0.39 (0.18) | 0.037 |
Energy production and conversion | ||||
ATP synthase subunit B | AIS04971.1 | NO | 5.50 (2.54) | 0.044 |
ATP synthase F0F1 subunit beta | AIS04975.1 | NO | 3.63 (0.48) | 0.002 |
Bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase | AIS06989.1 | NO | 3.16 (0.31) | 0.002 |
Isocitrate dehydrogenase | AIS07371.1 | NO | 4.57 (0.55) | 0.002 |
Electron transfer flavoprotein subunit beta | AIS07482.1 | YES | 5.65 (0.89) | 0.005 |
Dihydrolipoamide succinyltransferase | AIS07561.1 | NO | 2.44 (0.45) | 0.036 |
Fumarate reductase | AIS07564.1 | NO | 3.08 (0.59) | 0.010 |
Acetyl-CoA hydrolase | AIS08069.1 | NO | 4.74 (1.68) | 0.040 |
NADPH:quinone oxidoreductase | AIS08156.1 | NO | 15.42 (16.20) | 0.034 |
Dihydrolipoamide acetyltransferase | AIS08190.1 | YES | 3.08 (0.73) | 0.010 |
Unknown function | ||||
Hypothetical protein | AIS05430.1 | NO | 19.23 (19.19) | 0.001 |
Hypothetical protein | AIS05481.1 | YES | 0.17 (0.08) | 0.000 |
Unknown function | ||||
Hypothetical protein | AIS05936.1 | YES | 0.23 (0.13) | 0.006 |
DNA-binding protein | AIS07086.1 | YES | 0.24 (0.17) | 0.037 |
Hypothetical protein | AIS07091.1 | NO | 0.21 (0.12) | 0.000 |
DcaP-like protein | AIS07608.1 | YES | 11.07 (6.85) | 0.023 |
Glyoxalase | AIS07740.1 | YES | 0.30 (0.23) | 0.036 |
Hypothetical protein | AIS07873.1 | YES | 0.42 (0.16) | 0.011 |
Inorganic ion transport and metabolism | ||||
Hydroperoxidase | AIS06129.1 | YES | 0.35 (0.13) | 0.008 |
ABC transporter permease | AIS07421.1 | YES | 8.17 (3.60) | 0.034 |
Lipid metabolism | ||||
3-Oxoacyl-ACP reductase | AIS05519.1 | YES | 2.68 (0.94) | 0.038 |
Enoyl-CoA hydratase | AIS06112.1 | NO | 0.17 (0.24) | 0.004 |
Short-chain dehydrogenase | AIS06130.1 | YES | 0.10 (0.07) | 0.032 |
Nucleotide metabolism and transport | ||||
N5-carboxyaminoimidazole ribonucleotide mutase | AIS04832.1 | NO | 4.02 (2.98) | 0.010 |
Ribose-phosphate pyrophosphokinase | AIS05530.1 | NO | 4.61 (2.76) | 0.000 |
Deoxyuridine 5′-triphosphate nucleotidohydrolase | AIS05628.1 | NO | 0.28 (0.32) | 0.032 |
Orotidine 5′-phosphate decarboxylase | AIS06325.1 | NO | 0.19 (0.21) | 0.032 |
Adenylosuccinate lyase | AIS07333.1 | NO | 6.31 (2.68) | 0.047 |
Xanthine phosphoribosyltransferase | AIS07874.1 | NO | 2.42 (0.69) | 0.048 |
Inosine-5-monophosphate dehydrogenase | AIS08184.1 | NO | 3.94 (0.81) | 0.006 |
Post-translational modification, protein turnover, chaperone functions | ||||
Molecular chaperone DnaK | AIS04838.1 | NO | 2.13 (0.15) | 0.000 |
Post-translational modification, protein turnover, chaperone functions | ||||
Osmotically inducible protein C | AIS04957.1 | NO | 3.16 (1.14) | 0.029 |
Replication and repair | ||||
DNA repair protein | AIS04967.1 | YES | 3.87 (1.19) | 0.033 |
DNA polymerase I | AIS05372.1 | NO | 0.31 (0.34) | 0.038 |
Secondary metabolites: biosynthesis, transport and catabolism | ||||
mRNA 3′-end processing factor | AIS06680.1 | NO | 0.10 (0.19) | 0.027 |
Transcription | ||||
DNA-directed RNA polymerase subunit beta’ | AIS05081.1 | NO | 2.86 (0.42) | 0.025 |
Transcription elongation factor NusA | AIS05126.1 | NO | 3.53 (0.94) | 0.036 |
Transcription termination factor Rho | AIS05365.1 | NO | 3.60 (1.54) | 0.025 |
DNA-binding protein | AIS07045.1 | NO | 9.73 (6.82) | 0.029 |
DNA-directed RNA polymerase subunit alpha | AIS07896.1 | NO | 3.28 (0.46) | 0.001 |
Translation | ||||
Tyrosyl-tRNA synthetase | AIS04798.1 | NO | 3.37 (0.97) | 0.009 |
23S rRNA methyltransferase | AIS05117.1 | NO | 3.37 (4.80) | 0.037 |
Alanyl-tRNA synthetase | AIS05862.1 | NO | 3.05 (1.16) | 0.004 |
30S ribosomal protein S20 | AIS06347.1 | YES | 3.77 (2.31) | 0.026 |
Ribosome-recycling factor | AIS06861.1 | NO | 3.91 (1.42) | 0.045 |
Peptide chain release factor 1 | AIS07012.1 | NO | 0.34 (0.27) | 0.021 |
30S ribosomal protein S6 | AIS07031.1 | NO | 6.92 (1.66) | 0.000 |
30S ribosomal protein S18 | AIS07032.1 | NO | 22.70 (16.56) | 0.028 |
50S ribosomal protein L9 | AIS07033.1 | NO | 8.17 (3.02) | 0.002 |
30S ribosomal protein S2 | AIS07184.1 | NO | 3.87 (1.07) | 0.040 |
Translation | ||||
Tryptophanyl-tRNA synthetase | AIS07557.1 | NO | 8.09 (4.24) | 0.023 |
50S ribosomal protein L27 | AIS07585.1 | YES | 10.28 (8.73) | 0.027 |
Aspartyl-tRNA synthetase | AIS07787.1 | NO | 5.92 (1.79) | 0.038 |
30S ribosomal protein S9 | AIS07845.1 | YES | 5.86 (1.38) | 0.009 |
50S ribosomal protein L17 | AIS07895.1 | YES | 9.64 (4.81) | 0.003 |
30S ribosomal protein S4 | AIS07897.1 | NO | 13.30 (3.19) | 0.010 |
50S ribosomal protein L15 | AIS07902.1 | NO | 5.92 (1.17) | 0.000 |
30S ribosomal protein S5 | AIS07904.1 | YES | 4.61 (5.00) | 0.003 |
50S ribosomal protein L18 | AIS07905.1 | YES | 9.04 (10.58) | 0.033 |
50S ribosomal protein L6 | AIS07906.1 | YES | 7.59 (1.58) | 0.037 |
30S ribosomal protein S8 | AIS07907.1 | YES | 10.76 (2.98) | 0.011 |
50S ribosomal protein L5 | AIS07909.1 | NO | 7.73 (2.20) | 0.000 |
50S ribosomal protein L24 | AIS07910.1 | YES | 4.25 (0.70) | 0.038 |
50S ribosomal protein L14 | AIS07911.1 | NO | 11.07 (12.57) | 0.002 |
30S ribosomal protein S17 | AIS07912.1 | NO | 12.36 (3.91) | 0.048 |
50S ribosomal protein L16 | AIS07914.1 | YES | 29.11 (22.95) | 0.038 |
30S ribosomal protein S3 | AIS07915.1 | NO | 6.73 (3.82) | 0.026 |
50S ribosomal protein L22 | AIS07916.1 | NO | 1.89 (0.85) | 0.035 |
50S ribosomal protein L2 | AIS07918.1 | YES | 7.87 (3.98) | 0.038 |
50S ribosomal protein L23 | AIS07919.1 | YES | 4.45 (1.04) | 0.018 |
50S ribosomal protein L4 | AIS07920.1 | NO | 14.45 (7.02) | 0.002 |
Translation | ||||
50S ribosomal protein L3 | AIS07921.1 | NO | 6.25 (1.29) | 0.042 |