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Fig. 3 | BMC Genomics

Fig. 3

From: Clostridium sordellii genome analysis reveals plasmid localized toxin genes encoded within pathogenicity loci

Fig. 3

Southern hybridisation analysis of pulsed-field gels to confirm the presence of pCS1- and pCS2-type plasmids in C. sordellii strains. (A) A blot of a pulsed-field gel using C. sordellii genomic DNA-containing agarose plugs digested with BlpI (to linearise pCS1-type plasmids), hybridised with a tcsL specific probe; (B) the blot from panel A was stripped and reprobed with a probe specific for parB from pCS1-1; (C) a blot of a separate pulsed-field gel using purified uncut C. sordellii genomic DNA, hybridised with a probe specific for ATCC9714PCS2_00141 from pCS2. The migration of molecular size markers is indicated on the left, and the C. sordellii isolate corresponding to each lane is indicated above

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BMC Genomics

ISSN: 1471-2164

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