Fig. 7From: Deep sequencing-based characterization of transcriptome of trifoliate orange (Poncirus trifoliata (L.) Raf.) in response to cold stressqPCR analysis of differentially expressed genes in Poncirus trifoliata under low temperature. (a, b) Transcript levels of 17 randomly selected DEGs, including 12 up-regulated (a) and 5 down-regulated (b). The Y-axis on the left shows the relative gene expression levels analyzed by qPCR (red lines), while Y-axis on the right shows corresponding expression data of RNA-seq (gray histogram). The X-axis represents the time (hours) of 4 °C treatment. The bars represent SE (n = 3). (c) Comparison between the log2 of gene expression ratios obtained from RNA-seq data and qRT-PCRBack to article page