Table 2 Validation of microarray expression data by quantitative RT-PCR (qRT-PCR). Pearson correlation coefficients were calculated for microarray and qRT-PCR data for 12 gene transcripts. ICuGI indicates the International Cucurbit Genomics Initiative version 4.0 gene identifiers for each unigene (www.icugi.org)
Unigene Identifier | ICuGI | Gene name | Correlation |
|---|---|---|---|
cCL451Contig1 | MU46283 | 1-aminocyclopropane-1-carboxylate oxidase (CmACO1) | 0.911 |
cPSI_11-E04-M13R_c | MU64933 | N-acetyltransferase hookless1 (CmACT1) | 0.896 |
cHS_07-B10-M13R_c | MU61400 | DNAJ domain (CmDNAj) | 0.510 |
cCL6170Contig1 | MU54142 | Ethylene-responsive transcriptional coactivator (CmERT) | 0.943 |
cCI_54-H09-M13R | MU48499 | Ethylene receptor (CmETR1) | 0.371 |
cCL1086Contig1 | MU43941 | Expansin (CmEXP1) | 0.724 |
cCL212Contig1 | MU44581 | NAM like protein (CmNOR) | 0.931 |
cCL652Contig1 | MU46792 | ORANGE chaperone protein DNAJ-related (CmORG) | 0.882 |
MU10927 | MU50814 | Polygalacturonase (CmPG1) | 0.947 |
MU10942 | MU49862 | Phytoene synthase (CmPSY1) | 0.871 |
cA_27-A08-M13R_c | MU51300 | Terpenoid synthase (CmTER) | 0.914 |
cCL6092Contig1 | MU45836 | Xyloglucan endotransglucosylase/hydrolase (CmXTH1) | 0.962 |