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Table 3 Proteins whose expression were significantly higher expressed (+) in XZ5 compared with XZ54 leaves under control condition (XZ5 vs XZ54)AASC, Amino acid sequence coverage; Protein spot ID refers to numbers in Fig. 1. Accession number of top database match from the NCBInr database. Fold increase and decrease were calculated as drought/control, and –control/drought for up and down -regulated proteins respectively. All ratios shown are statistically significant (p < 0.05). +106 and -106 referred to the specific expressed and totally inhibited proteins, respectively

From: Comparative proteomic analysis of drought tolerance in the two contrasting Tibetan wild genotypes and cultivated genotype

Spot No. Protein name C. I. (%) Accession number MW (Da) pI AASC (%) Fold increase (+) or decrease (-)
XZ5 vs XZ54a XZ5b XZ54 ZAU3 Function
  Nine day drought stress (SMC 10 %)           
A3 Melanoma-associated antigen p97 [G. gallus] 96.2 gi|45383930 80860 5.9 22.0 +106 1.7 NE −106 Stress response
(C2)c
A4 Type I chlorophyll a/b-binding protein b [A. tricolor] 100 gi|13676406 16608 4.6 18.8 +106 5.6 NE NE Photosynthesis
(B1C3)
A6 Glutathione S-transferase 1 [T. aestivum] 100 P30110|GSTF1_WHEAT 25811 5.3 29.3 +106 +0.6 NE −1.7 Stress response
A7 Ribulosebisphosphate carboxylase large chain (RuBisCO large subunit) [W. mirabilis] 99.2 RBL_WELMI 49579 6.3 25.1 +106 +0.5 NE +2.2 Photosynthesis
A12 Ribulosebisphosphate carboxylase/oxygenaseactivase B (RuBisCOactivase B) [A. thaliana] 99.4 gi|10720253 47199 7.6 24.9 +106 −0.1 +106 −1.2 Photosynthesis
A13 Putative ankyrin repeat domain protein 2 [O. sativa] 100.0 gi|108712139 37253 4.6 10.0 +29.6 +1.4 +0.1 −1.9 Metabolism
A14 Transketolase,chloroplast [Z. mays] 99.9 Q7SIC9|TKTC_MAIZE 72948 5.5 15.1 +106 +1.6 +106 −2.3 Photosynthesis
(B2)
  Twenty day drought stress (SMC 4 %)           
B4 Putative mono-dehydroascorbate reductase, Os09g0567300 [O. sativa] 100 gi|115480733 46644 5.5 17.9 +7.3 +1.2 +2.3 +1.1 Stress response
B5 Elongation factor G [A. thaliana]] 100 gi|62320532 47645 5.45 21.4 +1.8 −0.03 −1.4 −0.5 Amino-acid biosynthesis
  Two days of recovery           
C10 Putative ascorbate peroxidase [T. aestivum] 100 gi|25992559 39922 5.5 5.5 +1.6 +1.8 +1.8 −1.3 Stress response
C11 Heat-shock protein [Secale cereale] 100 gi|556673 88063 4.9 8.2 +2.3 +0.1 −0.1 −2.5 Stress response
C12 Ribulose bisphosphate carboxylase large chain (RuBisCO large subunit) [W. mirabilis] 99.2 RBL_WELMI 49579 6.3 25.1 +106 −0.1 +106 +1.7 Photosynthesis
  Five days of recovery           
D6 Putative mono-dehydroascorbate reductase, Os09g0567300 [O. sativa] 100 gi|115480733 46644 5.5 17.9 +3.2 +0.2 −0.1 +1.4 Stress response
  1. a Fold increase under control condition in XZ5 vs XZ54
  2. b Fold increase or decrease in XZ5, XZ54 and ZAU3 (drought vs control)
  3. c the same protein spots identified at different stages