Fig. 1

PCA plots of C. jejuni transcriptomic samples. The Log(RPKM + 1) values for each gene in the C. jejuni NCTC11168 genome were used for PCA analysis and PC1vsPC2 (a) and PC1vsPC3 (b) were plotted to show the overall structure of the transcriptomic data and to identify sample outliers. Each dot represents a RNA-seq sample with iron-replete samples in dark colors and iron-limited samples in light colors. The PCA showed distinct sample separation based on the iron status of the growth medium highlighting the key influence of iron on the C. jejuni transcriptome. The samples for each strain tend to cluster together with two noticeable exceptions in the ΔperR iron-replete and Δfur limited groups