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Fig. 11 | BMC Genomics

Fig. 11

From: Characterization of a second secologanin synthase isoform producing both secologanin and secoxyloganin allows enhanced de novo assembly of a Catharanthus roseus transcriptome

Fig. 11

CDF97 performance in quality reconstruction and transcript expression level. a Current resources (A = ccOrcae, B = mpgrCra, C = NIPGR, D = PMS454, E = PMSIllu), CD97 and CDF97 were used as databases to identify homologs of MIA genes, and the resulting bitscore obtained by BLAST was compared to that of an ideal sequence (bitscore of the reference sequence against itself, i.e. bitscore ratio = 1). Values inside each cell indicate the number of hits having a ratio >0.8. b Comparison of log2 expression levels measured by qPCR and RNA-seq for SLS and T16H isoforms in current assemblies, CD97 and CDF97. RNA-seq samples used for this comparison were: flowers (Fl, SRR122239 and SRR1271859), mature leaves (ML, SRR122251 and SRR1271857), roots (R, SRR122254 and SRR1271858) and young leaves (YL, SRR122252). Expression levels were normalized by RPS9, in both qPCR and RNA-seq measurements. The linear regression line is shown in blue with shading corresponding to 95 % confidence intervals. The r² values indicate correlation coefficients of the linear models

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