Fig. 3

The pneumocyclicin cassette putatively encoding a newly-identified circular bacteriocin within the pneumococcal population. a. Comparison of the gene organisation of the pneumocyclicin cassette with those of class IIc(i) circular bacteriocins AS-48, carnocyclin A, circularin A and uberolysin. The genes comA and comB, adjacent to but not considered to be part of the pneumocyclicin cassette, were included to demonstrate that the synteny with other circular bacteriocin cassettes extends beyond the minimal cassettes and includes the downstream ABC transporter genes. b. Comparison of the pneumocyclicin precursor peptide sequence and predicted secondary structure with that of other class IIc(i) circular bacteriocins. Figure adapted from Martin-Visscher et al. [35]. c. Neighbour-joining tree demonstrating two distinct clusters of alleles representing the 115 pneumocyclicin cassettes in the study dataset