Fig. 5

RT-PCR analysis of the gene expression from the predicted cys pathway, ssu1 and ssu2 operons of X. citri. (a) Genes from the predicted cys regulon; (b) genes from predicted operons ssu1 and (c) ssu2. Total RNA was extracted from cells grown in LB and XAM1 media during mid end-exponential growth phase. RT-PCR was carried out as described in the Methods. gDNA: genomic DNA (100 ng); cDNA: complementary DNA (200 ng); RNA: negative control. Genes are described according the number followed by the correspondent name as shown in KEGG data bank. Proteins encoded by the genes showed with asterisk (*) had the expression identified in the proteomic analysis of X. citri extracts obtained after growth in XAM1 medium [10]. Blankets group genes that belong to the same predicted operons. As a positive control a 271 bp fragment of the 16S rRNA is shown. The arrows indicate the expected molecular mass for each transcript