Fig. 6

Expression levels of four conserved and three novel miRNAs between two flowering phases with significance in qRT-PCR. Based on RNA-seq results, 12 significantly different expressed miRNA were exposed to qRT-PCR assay for validation, which resulted in seven validated miRNA. Each experiment consisted of three biological and technical reps. The relative expression levels of the selected genes were calculated using the 2^–ΔΔCT method, and the significant threshold was set at 0.01 (**: <0.01). The U6 gene was used as a Control. Error bars represent the standard deviation of the mean expression values. At PS4, novel-miR11, novel-miR31, novel-miR37 and cen-miR396a-5p were down-regulated, while cen-miR160a-5p, cen-miR394 and cen-miR535-5p were up-regulated