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Fig. 2 | BMC Genomics

Fig. 2

From: Experimental validation of methods for differential gene expression analysis and sample pooling in RNA-seq

Fig. 2

Agreement between sequencing RNA-pools and sequencing corresponding individual RNA samples. a Intersection between differentially expressed genes (DEGs), detected by edgeR, in RNA-seq data from pooled RNA (3 samples/ pool; two pools/ group) and of data from corresponding individual samples of RNA (3 samples/group). Rectangle represents all expressed genes. b Correlation between the logarithmic (base 2) fold changes (LFC) in expression that were estimated by sequencing RNA-pools (3 samples/ pool) and by sequencing corresponding individual samples (3 samples/group). c Intersection between the DEGs, detected by edgeR, in RNA-seq data from pooled RNA (8 samples/ pool; two pools/ group) and of data from corresponding individual samples of RNA (8 samples/group). Rectangle represents all expressed genes. d Correlation between the LFC in expression that were estimated by sequencing RNA-pools (8 samples/pool) and by sequencing corresponding individual samples (8 samples/ group)

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BMC Genomics

ISSN: 1471-2164

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