Fig. 7

Validation of the differential abundance of Lhx2 LongSAGE tags in Wt vs. Nr2e1 ^frc/frc embryos. a The tag count results, mapping to Lhx2 at the three different embryonic days, are presented. Columns one to three present the tag sequence, accession number, and gene symbol corresponding to Lhx2; columns four to six, the corrected tag numbers recovered from DiscoverySpace in both Wt and Nr2e1 ^frc/frc embryos at each time point (E13.5, 15.5, and 17.5); column seven, the fold change between the tag numbers corresponding to Lhx2 found in Wt and Nr2e1 ^frc/frc embryos at each time point; and column eight, the associated P values obtained using the Audic-Claverie statistical method. According to this approach, Lhx2 expression level is significantly upregulated at both E13.5 and E15.5 in Nr2e1 ^frc/frc embryos. b Wt embryonic stem cells (ESC) submitted to a neurogenic differentiation protocol demonstrated expression of Nr2e1 at 12 days of differentiation (d12) whereas, as expected, Nr2e1 ^frc/frc ESC did not express Nr2e1 (*, P < 0.001). c Quantitative RT-PCR reveals that the Lhx2 mRNA level is upregulated by ~3.6 fold in Nr2e1 ^frc/frc ESC at d12 compared to Wt ESC (*, P < 0.01). d Immunofluorescence using an anti-Lhx2 antibody (green) demonstrated a similar expression pattern for the Lhx2 protein along the ventricular/subventricular zone (VZ/SVZ) of the lateral telencephalon in E15.5 Nr2e1 ^frc/frc embryos compared to Wt. White arrows, medial pallium; red arrows, dorsal pallium; scale bar, 200 μm. e Lhx2 protein level was increased by ~1.3 fold along the VZ/SVZ of the lateral telencephalon in E15.5 Nr2e1 ^frc/frc embryos compared to Wt (*, P < 0.05). (c), (d), (e) Sample Student’s t-test were performed, N = 3; error bars, standard error of the mean