Skip to main content

Table 2 Relative transcript levels of Puccinia genes in wheat seedlings infected with VIGS constructs compared to seedlings infected with the control BSMV virus

From: Identification of promising host-induced silencing targets among genes preferentially transcribed in haustoria of Puccinia

Gene ID

Relative transcript levels of target gene in HIGS assaysa

 

Puccinia spp. Testedb

Leaf 1

Leaf 2

Leaf 3

Leaf 4

Leaf 5

Leaf 6

Averagec

 

PGTG_03590

0.55 ± 0.10

0.46 ± 0.09

0.32 ± 0.06

0.36 ± 0.07

0.49 ± 0.10

0.37 ± 0.08

0.425 ± 0.09

Pgt

PGTG_01136

0.79 ± 0.34

0.65 ± 0.27

0.25 ± 0.11

0.40 ± 0.17

0.67 ± 0.28

0.76 ± 0.32

0.59 ± 0.21

Pgt

PGTG_01215

0.47 ± 0.18

0.49 ± 0.19

0.36 ± 0.14

0.82 ± 0.32

0.58 ± 0.22

3.01 ± 1.16

0.96 ± 1.02

Pgt

PGTG_12890

0.21 ± 0.04

0.72 ± 0.14

0.77 ± 0.14

0.45 ± 0.09

0.54 ± 0.11

0.52 ± 0.10

0.54 ± 0.20

Pgt

PGTG_03478

0.59 ± 0.26

0.14 ± 0.06

0.72 ± 0.31

0.65 ± 0.28

0.81 ± 0.35

 

0.58 ± 0.26

Pgt

PGTG_10731

0.68 ± 0.14

1.44 ± 0.29

0.57 ± 0.12

0.68 ± 0.14

1.33 ± 0.27

2.45 ± 0.50

1.19 ± 0.72

Pgt

PGTG_14350

0.25 ± 0.08

0.62 ± 0.21

0.13 ± 0.04

0.25 ± 0.09

0.74 ± 0.25

1.96 ± 0.67

0.66 ± 0.68

Pgt

PGTG_01304

0.43 ± 0.09

0.76 ± 0.16

0.73 ± 0.15

0.37 ± 0.08

0.84 ± 0.18

0.64 ± 0.13

0.63 ± 0.19

Pgt

PSTG_04507

0.33 ± 0.18

0.23 ± 0.13

0.01 ± 0.01

0.40 ± 0.22

0.72 ± 0.40

3.54 ± 1.96

0.87 ± 1.33

Pst

PSTG_11830

0.58 ± 0.08

0.63 ± 0.09

0.38 ± 0.05

1.39 ± 0.19

0.95 ± 0.13

0.92 ± 0.13

0.81 ± 0.36

Pst

PSTG_04871

0.68 ± 0.10

0.87 ± 0.13

0.41 ± 0.06

0.69 ± 0.11

0.40 ± 0.06

1.64 ± 0.25

0.78 ± 0.46

Pst

PSTG_03360

0.76 ± 0.16

0.68 ± 0.14

0.67 ± 0.14

0.71 ± 0.15

0.57 ± 0.12

1.02 ± 0.22

0.74 ± 0.15

Pst

PSTG_04507

0.28 ± 0.26

0.46 ± 0.41

0.05 ± 0.04

0.58 ± 0.52

0.19 ± 0.18

0.26 ± 0.24

0.30 ± 0.19

Pt

PSTG_04871

0.57 ± 0.09

0.66 ± 0.11

0.71 ± 0.12

0.74 ± 0.12

0.71 ± 0.11

0.61 ± 0.10

0.67 ± 0.07

Pt

PSTG_03360

0.67 ± 0.35

0.65 ± 0.34

0.41 ± 0.21

0.10 ± 0.05

0.62 ± 0.32

 

0.49 ± 0.24

Pt

  1. aValues represent the average ratio of transcript levels from the VIGS challenged seedling leaf to transcript levels in six individual seedlings infected with the control virus, and the standard deviation of these six ratios
  2. bPGTG genes were infected with Pgt and Pgt transcript levels were measured. PSTG genes were infected with either Pst or Pt
  3. cAverage and standard deviation of the estimates of relative transcript levels of each of the individual plants in one HIGS assay
  4. The effect of the PGTG-Iaam construct on gene expression was examined previously [21]
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us