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Table 1 Statistics of reads and alignment to reference genome for four samples’s exome sequencing on TargetSeq-Proton/SureSelect-HiSeq platform

From: Comparison and evaluation of two exome capture kits and sequencing platforms for variant calling

Sample Sequencing platform Total reads (M) Total bases (Gb) Total mapped reads (M) Average read length (bp) Average coverage depth Coverage at 1× (%) Coverage at 5× (%) Coverage at 10× (%) Coverage at 20× (%)
S1 TargetSeq-Proton 48.2 4.5 42.9 94.1 39.8 97 94 90 79
SureSelect-HiSeq 78.3 7.8 77.4 2*100 40.0 100 97 90 71
S2 TargetSeq-Proton 62.4 6.1 55.1 97.4 51.9 98 95 93 86
SureSelect-HiSeq 79.9 7.9 79.1 2*100 45.3 100 98 91 74
S3 TargetSeq-Proton 61.3 5.5 55.3 90.9 50.3 97 94 91 84
SureSelect-HiSeq 56.8 5.6 56.2 2*100 33.4 100 96 86 63
S4 TargetSeq-Proton 53.9 5.2 49.4 96.9 49.8 97 94 91 84
SureSelect-HiSeq 59.5 5.9 58.9 2*100 36.0 100 96 87 66