Table 1 Statistics of reads and alignment to reference genome for four samples’s exome sequencing on TargetSeq-Proton/SureSelect-HiSeq platform
From: Comparison and evaluation of two exome capture kits and sequencing platforms for variant calling
Sample | Sequencing platform | Total reads (M) | Total bases (Gb) | Total mapped reads (M) | Average read length (bp) | Average coverage depth | Coverage at 1× (%) | Coverage at 5× (%) | Coverage at 10× (%) | Coverage at 20× (%) |
|---|---|---|---|---|---|---|---|---|---|---|
S1 | TargetSeq-Proton | 48.2 | 4.5 | 42.9 | 94.1 | 39.8 | 97 | 94 | 90 | 79 |
SureSelect-HiSeq | 78.3 | 7.8 | 77.4 | 2*100 | 40.0 | 100 | 97 | 90 | 71 | |
S2 | TargetSeq-Proton | 62.4 | 6.1 | 55.1 | 97.4 | 51.9 | 98 | 95 | 93 | 86 |
SureSelect-HiSeq | 79.9 | 7.9 | 79.1 | 2*100 | 45.3 | 100 | 98 | 91 | 74 | |
S3 | TargetSeq-Proton | 61.3 | 5.5 | 55.3 | 90.9 | 50.3 | 97 | 94 | 91 | 84 |
SureSelect-HiSeq | 56.8 | 5.6 | 56.2 | 2*100 | 33.4 | 100 | 96 | 86 | 63 | |
S4 | TargetSeq-Proton | 53.9 | 5.2 | 49.4 | 96.9 | 49.8 | 97 | 94 | 91 | 84 |
SureSelect-HiSeq | 59.5 | 5.9 | 58.9 | 2*100 | 36.0 | 100 | 96 | 87 | 66 |