Fig. 5

Multiple protein sequence alignments showing strain-specific differences in P1 and ORF6. Type1 and 2 strain names are highlighted in blue and green, respectively. a A poly-serine repeat in P1 with varying lengths in various strains. 19294 has a uniquely long allele of the poly-serine repeat, and the repeat-length in the other strains does not strictly correspond to strain type. b A stop-loss mutation in MAC results in an additional 7 amino acids added to the protein sequence. c A pair of frameshifts results in the truncation of the beginning of ORF6 in MAC. Type1 and Type2 are representative sequences for type 1 and other type 2 strains, respectively. The new protein is predicted to use an alternate start codon by RAST: the starting methionine in MAC is the same codon as that which codes for the leucine in other type 1 and type 2 strains