Table 2 Assessment of Cryptosporidium preparation from 17 stool samples containing C. hominis or C. parvum oocysts

Sample

Species and gp60 subtype

qPCR 18S rRNA C_T

Number of oocysts counted

Visual condition of oocysts

qPCR 18S rRNA C_T

Calculated genome copies

Mass of DNA (ng)

>1 ng DNA available for library preparation (isolate reference number)

1

C. hominis IaA14R3

ND

1.9×10⁷

Good

17.7

5.53×10⁷

77

Yes (UKH4)

2

C. hominis IbA10G2

23.5

1.6×10⁷

Good

20.0

1.24×10⁷

49.55

Yes

(UKH5)

3

C. hominis IdA18

29.3

2.1×10⁵

Good

25.7

1.64×10⁵

Below threshold

No

4

C. parvum IIaA15G1R1

31.6

7.5×10⁴

Good

25.9

1.49×10⁵

0.00

No

5

C. parvum IIaA15G1R2

31.1

5.0×10⁴

Good

25.1

2.36×10⁵

0.00

No

6

C. parvum IIaA15G2R1

28.1

3.6×10⁵

OK - a few empty oocysts, didn't stain well

28.6

2.66×10⁴

0.00

No

7

C. parvum IIaA18G2R1

29.1

1.5×10⁶

Good

22.2

2.99×10⁶

5.45

Yes

(UKP3)

8

C. parvum IIaA15G2R1

25.5

2.8×10⁶

Good

20.1

5.99×10⁶

18.65

Yes

(UKP4)

9

C. parvum IIaA15G2R1

27.5

9.1×10⁵

Good

23.2

1.52×10⁶

2.68

Yes

(UKP5)

10

C. parvum IIaA15G2R1

27.1

2.7×10⁶

Good

23.4

1.32×10⁶

4.40

Yes

(UKP6)

11

C. parvum IIaA17G1R1

23.8

9.0×10⁶

Good

20.3

1.01×10⁷

28.65

Yes

(UKP7)

12

C. parvum IIaA19G2R1

30.4

1.1×10⁶

Good

24.4

3.74×10⁵

0.8

No

13

C. parvum IIaA19G2R1

29.2

1.1×10⁶

Good

28.7

2.53×10⁴

0.00

No

14

C. parvum IIaA19G2R1

30.6

5.2×10⁵

OK

31.8

3.58×10³

0.00

No

15

C. parvum IIaA19G2R1

34.0

3.9×10⁴

OK - a few empty oocysts

No amplicon detected

0

ND

No

16

C. parvum IIdA22G1

28.4

2.3×10⁶

Good

21.9

3.62×10⁶

6.55

Yes

(UKP8)

17

C. parvum IIdA24G1

30.9

1.2×10⁶

Good

23.9

1.01×10⁶

2.88

Yes^a