Fig. 1
User Interface Snapshots. a The instructions for generating the input data format, including each column description is described in “Step #1” in the PhosphOrtholog main page. The input interface also shows an example of the required data format in the table below the text “For example”. The data in the example table can be used as input by clicking the “Use above example” button. Mapping of this data can be completed by clicking “Map”. Input data can also be simply copy-pasted/edited/deleted on the user interface (UI) spreadsheet like with an Excel spreadsheet in the “Preview for input data set” table. Three separate example input files can also be downloaded through the ‘download’ links immediately below the example data table and uploaded to the UI through the “Upload” button. User provided datasets (in comma-delimited format) can be uploaded for mapping via the “Upload” button/copy-pasted into the preview input table or typed in. b Output Interface: Once mapping is ensued with the ‘Map’ button in “Step # 2”, the progress bar above the output table in “Step # 3” tracks the progress of the mapping function. This will give a rough estimate of how long the job will take to finish for large data sets. The first two columns in the mapped output table indicates the species 1 record identifier and PTM site details which is mapped to the orthologous species 2 site information shown in the third and fourth columns. The last column indicates the E-value significance score from the pairwise sequence alignement of the orthologous proteins. If the PTM site is a known mapped site from PhosphoSitePlus database, then this column reports “From PhosphoSitePlus” instead of a E-value. Once mapping is complete, this bar also reports the number of novel sites mapped by PhosphOrtholog, the percentage of novel sites that could be mapped in the data set and the percentage of known sites from PhosphoSitePlus that could be recovered by PhosphOrtholog