Candidate effectors contribute to race differentiation and virulence of the lentil anthracnose pathogen Colletotrichum lentis

Table 4 ClToxB homologs

Toxin B homologs	Pathogen	GenBank	Isolate/Strain	Peptide (aa)	SP^a	MP^b	Identity \|Similarity^c
ClToxB	Colletotrichum lentis	JZ350031	CT-21 (Race 1) & CT-30 (Race 0)	94	19	75	100 \| 100
Ptrtoxb	Pyrenophora tritici-repentis	AAM00019	SD20 (Race 4)	88	23	65	40 \| 50
PtrToxB	Pyrenophora tritici-repentis	AF483831	Alg3-24 (Race 5)	87	23	64	39 \| 53
CgToxB	Colletotrichum gloeosporiodes	ELA28482	Nara gc5	86	19	67	44 \| 57
ChToxB	Colletotrichm higginsianum	CCF45936	IMI349063	87	19	68	52 \| 66
CoToxB	Colletotrichum orbiculare	ENH84621	104-T	87	19	68	44 \| 58
MoToxB	Magnaporthe oryzae	ELQ42910	Y34	88	20	68	36 \| 52
Ptrtoxb	Pyrenophora tritici-repentis	AF483832	90-2 (Race 4)	80	15	65	40 \| 50

An E value cut-off of 1e-6 was used to extract homologs of ClToxB from NCBInr protein database. All homologs contain four characteristic cystine residues, which are likely to form 2 disulfide bonds. ^aSP, Signal Peptide (predicted using SignalP server version 4.1)^bMP, Mature Protein^cIdentity/Similarity (in per cent) as calculated by Needleman-Wunsch pairwise alignment of ClToxB (MP) with its homologs.

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